The Protein Man's Blog | A Discussion of Protein Research

How Chaperone-Assisted Protein Folding Works

The Amazing Origins of Antibody Diversity

How to Prepare Samples for Western Blot Analysis

The era of messenger RNA vaccines

Guess what??? Immobilized Papain Generates Fab Fragments of Cluster A Monoclonal Antibodies for Validation of Inner Domain of HIV-1 gp120?

What is the Link between Protein Folding and Prion Disease?

Exploring the Genetic Causes of ALS (Lou Gehrig's Disease)

Fluorescence detection of proteins in Western blotting

Cells of the Immune System

How the cilium became a ‘villain’ in Apicomplexan parasites?

Molecular Beacon: Dual Labelled Probes

Subcellular Fractionation: ‘Exploring cells through centrifugation'

Exposure to Intestinal microbes in early life can influence development of thymic lymphocytes : A review of current literature

Antibody Basics Revisited (Do’s and Don’ts of Antibody Handling)

The Use of Spectral Scanning in Nucleic Acid Purity Assessment

CAR T-Cells, a New Solid Tumor Therapy Breakthrough?

Spotting Fragmentation Patterns When Using Mass Spectrometry

How to Calculate Protein Concentration Using The Bradford Assay

The Importance (and Implications) of Optogenetics

What's the Difference Between lncRNA and lincRNA?

CRISPR, ZFNs, TALENs: Differences Between Bioengineering Technologies

Antibody Purification by Affinity Chromatography

How the ACE2 Receptor Enables SARS and COVID-19

Why Do I Need a Cocktail for Proteases and Phosphatases?

LDH Cytotoxicity Assay FAQs

What should you consider when selecting a protein extraction buffer?

Plasmid Isolation: Overcoming the Challenges for Isolating Plasmid DNA

Taq Polymerase is Preferred Enzyme for Polymerase Chain Reaction (PCR)

The Role of Tween 80 in Protein Solubilization and Stabilization

Blocking Buffer Selection Guide: Tips for Selecting the Best Blocking Agent

Tips for Using Ni-NTA Resin

Why RIPA is for More Than Just Radioimmunoprecipitation Assays

Trypsin & Its Role in Protein Analysis

Capture and purification of low abundance proteins

How to Get Accurate Results with Affinity Purification and Affinity Chromatography

How to Prepare Samples for Western Blot Analysis | G-Biosciences

What’s the Difference Between Tween 20 and Tween 80? | G-Biosciences

The Role of LDH in Cellular Cytotoxicity

Bradford Assay for Protein Concentration | G-Biosciences

Purify Proteins Fast: How FPLC Speeds the Process

The Benefits of a Protease Inhibitor Cocktail

Protein palmitoylation and sulfhydryl chemistry methods to capture palmitoylated proteins

Complete Protease Inhibitor Cocktails and How They Work

The Difference Between the BCA and Bradford Protein Assays

Protein Kinases and Phosphatases: drivers of phosphorylation and dephosphorylation

Protein phosphorylation: Nature’s favorite way of decorating proteins

Click chemistry and its application to proteomics

Why Is Bovine Serum the Preferred Standard for Protein Assays?

How to Select the Best Protein Assay

DNFB-Sanger’s reagent for detection of free amino acids

How to Iodinate Proteins with Iodo-Gen®

Hot Start PCR: An Overview

Use of Chaperones in Recombinant Protein Expression

Using Western Transfer for Protein Detection: Some Commonly Used Methods

The Basics of Genomic DNA Extraction

Iodination of Proteins with Bolton-Hunter Reagent

Multiplex PCR, Benefits over traditional PCR

Best Methods to Stain Proteins After Protein Electrophoresis

Dyes, Ions, or Fluorescent Stains: What Are the Best Ways to Visualize Protein In Gels?

Best Ways to Remove Detergents in Protein Samples

Different Types of Extraction Buffers and When to Use Them

Preventing Carbamylation When Using Urea as a Protein Solubilization Buffer

8 Factors to Consider when Selecting a Protein Cross-linker

BCA or Bradford Protein Assay: Choosing Between the Two

Four Methods for Endosomal Isolation

Molecular Beacons: Dual Labeled Probes

Mycoplasma Contamination in Cell Cultures: Detection & Prevention

Antibody Selection for Immunohistochemistry

Protein Purification with IMAC

A Guide to Sample Preparation for Immunofluorescence

Detection of Apoptosis by TUNEL Assay

Luciferase Reporter Assays: An Overview

Protein Estimation Methods

Host Cell Protein Analysis: Detection in Biotherapeutics

Tips for Preventing Protein Aggregation & Loss of Protein Solubility

How to set up a teaching biotech lab

How to Lyse Bacterial Cells

Why Start a High School Biotech Course?

A Quick Guide to Choosing Fluorescent Dyes for Protein Labeling

Biotech Education: Good for Students, Teachers & All

TOP 5 REASONS FOR A COLLEGE BIOTECH PROGRAM

Three most common ELISA Challenges

The Most Basic Biotech Lab Equipment, Materials & Necessities

Physical Disruption or Chemical Cell Lysis? How to Decide

Organic Solvent Extraction of Proteins

Specificity of Immunostaining methods: How to choose a control?

Sample preparation for Mass spectrometric analysis

Blue Native Polyacrylamide Gel Electrophoresis: A simple tool for characterization of Protein Complexes

Strategies to stabilize aggregate-prone proteins in E.coli

Tips for Selecting Carrier Proteins

Role of additives in cell lysis

A Quick Overview of Phenol Extraction

Microfluidic 3D cultures: An advanced tool to study the cultures at a single-cell level

Tris Tricine SDS PAGE: What is it and how to PERFORM it?

Contract Manufacturing Solutions for Biotech & Life Sciences

cGMP: What is it and what are the benefits?

Detergent Screening For Membrane Protein Extraction: What To Choose?

Ride the GeneCAPSULE & Explore Our Story!

Membrane Protein Extraction: The Basics

A Tutorial for 3D Cell Culture System: Bridging the Gap between in-vitro and in-vivo cultures

Molecular diagnostics- Lateral flow assay

A guide to in-vitro cultures: which matrix to be considered

Loop Mediated Isothermal Amplification (LAMP)

Genome-wide Ribosome footprint profiling

An Overview of Histology Fixatives

Why teach biotechnology in schools?

Methods of Determining Protein Stability

The Advantages of Using Trypsin for Mass Spectrometry

Antibody Labeling: Which Method Should You Use?

Protein Tags: How to Choose?

8 Techniques to Clone A Gene: Which Method is THe BEst FOr You?

Post Translational Modifications: what expression system to choose?

The Advantages of Biotinylation Tagging in Protein Purification

The Basics of Affinity Purification/Affinity Chromatography

Restriction Enzyme Analysis: How to Make the Cut

Proteomic Grade Detergents: Why you should use them

Exosome isolation by centrifugation, filtration, immunoaffinity & more

Multiplex Immunohistochemistry

Protein Solubility & Refolding Active Proteins from Inclusion Bodies

The Sandwich ELISA: Process and Practical Applications

How to Improve Spot Resolution During 2D-Electrophoresis

Recombinant Protein Expression Systems: Pros & Cons

Cold-Shock Keeps Recombinant Proteins out of Inclusion Bodies

BONCAT (Bioorthogonal Non-canonical Amino Acid Tagging): a method to define the translatome

Basics of Using Cytology for Cancer Testing

SILAC: A General Workflow for Improved Mass Spectrometry

SILAC for Improved Mass Spectrometry & Quantitative Proteomics

Key Differences of H&E and Special Stains for Immunohistochemistry

Study Protein to Protein Interaction with Protein Cross Linking to Glass

When to Use Immunohistochemistry (IHC) Detection vs. a Direct Method

Traction Force Microscopy for cell interaction with extracellular matrix

Immunoprecipitation Protocol: Critical Parameters

Immunoprecipitation Protocol: The Basics

MTT Assay for Cytotoxicity

Protein Storage For Protein Stability And Less Protein Degradation

Is Your BCA Protein Assay Really the Best Choice?

Remove BSA & Gelatin from Antibody Solutions for Antibody Labeling

Bacterial Growth Curves using a Spectrophotometer (Turbidimetric Determination)

Speed Up Protein Purification with FastPure™ Spin Columns

Overcome effects of detergents & reducing agents in protein estimation

Chromatography Series Part I: Liquid Chromatography Basics

How to isolate membrane receptor complexes from cells

How much biotin is coupled to my protein? The HABA/Avidin Assay

Sputum Processing, from viscous mucus to homogenous samples

Role of reporter genes to assay for transcription factors & more

High Efficiency & Stability Protein CrossLinking with EDC & NHS

Role of Non-Detergent Sulfobetaines in Protein Purification

Ammonium Sulfate Protein Precipitation: The key to Salting-Out

Modifying Oligonucleotide 5'-Phosphates By EDC for Improved Coupling

Optimize Elution Conditions in Affinity Chromatography to Antibodies

Immunoprecipitations (IP) for Protein Purification & Interactions Studies

How to select the Right protease inhibitor

Protect proteins with cryoprotectants & protein concentration

ELISA Blocking Agents & Blocking Solutions

Optimize Antigen and Antibody Concentration for Western Blots

Advantages of Magnetic Beads for Protein Immunoprecipitation

Detergents and their role in successful SDS-PAGE electrophoresis

Polyclonal Antibody Advantage for Antigen and Epitope Polymorphism

Genomic DNA Extraction: Plasmid and Genomic DNA Isolation Differences

Mitochondrial DNA from Fungi (yeast) via Spheroplasts

CNBr-activated resin to immobilize ligands for affinity chromatography

Western Blot Blocking: Tips and Tricks for Blocking Agents

The Differences between a Protoplast and a Spheroplast (And Their Subsequent Uses)

Cellulase, a lytic enzyme to break plant cell walls for protein extraction

Stripping Membranes: A Great Addition to Western Blot Protocols

ECL (Enhanced Chemiluminescence) Reagents: Enhanced How?

Key Protein Concentration & Enrichment Techniques | G-Biosciences

Importance of detergent micelle levels in membrane protein purification

Epoxy-activated resin: a versatile affinity chromatography support

Plant Protein Extraction and Protein Precipitation Techniques

Specific plant tissue sample preparation & protein extraction methods

Cell Disruption Techniques: Sonication, Dounce Homogenizer & More

Non-Specific Binding: Why It Needs to be Blocked in Western blots!

Precautions with SDS Stock Solutions Preparation | G-Biosciences

Western Blot Blocking: Tips and Tricks

Cell Lysis: 5 Common Cell Disruption Methods | G-Biosciences

Detergents: Ionic, Non-Ionic, and Zwitterionic. What's the Difference?

Buffer Solution Preparation: An Essential Skill for Researchers

Plant vs Animal Cells: Break the Cell Wall with Lytic Enzymes

Western blot: Constant current or constant voltage; which is best?

Best Blocking Buffer Selection for ELISA & Western Blot

Polyclonal Antibody: Advantage over Monoclonals?

Viability Assays: Different Types and Their Use in Cell Death Research

What is Protein Electrophoresis and When Should It Be Used?

A Derivatizing Agent for Glutathione Assays

Glutathione Assays To Monitor Oxidative Stress

The Advantages of Loading Controls in Western Blotting

Tips for Preventing a High Background During Western Blotting

The Top Methods for DNA Denaturation

What is Drop Dialysis: An Overview

How to Ensure Proper Protein Transfer to a Membrane

Role of Lytic Enzymes in Protein and DNA Extraction

How to protect proteins during protein extraction

Coupling of ligands for carbohydrate affinity chromatography

Gel Filtration and Its Role in Desalting and Buffer Exchange

Fast Flow Agarose Resins: What's The Real Difference?

Applications of important lectins

How to Determine the Chemical Solubility of a Product

ELISA Substrates: A Selection Guide

Common Subcellular Markers You Need to Know About

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium