The Protein Man's Blog | A Discussion of Protein Research

Why RIPA is for More Than Just Radioimmunoprecipitation Assays

Trypsin & Its Role in Protein Analysis

Capture and purification of low abundance proteins

How to Get Accurate Results with Affinity Purification and Affinity Chromatography

How to Prepare Samples for Western Blot Analysis

What’s the Difference Between Tween 20 and Tween 80?

The Role of LDH in Cellular Cytotoxicity

Bradford Assay for Protein Concentration

Purify Proteins Fast: How FPLC Speeds the Process

The Benefits of a Protease Inhibitor Cocktail

Protein palmitoylation and sulfhydryl chemistry methods to capture palmitoylated proteins

Complete Protease Inhibitor Cocktails and How They Work

The Difference Between the BCA and Bradford Protein Assays

Protein Kinases and Phosphatases: drivers of phosphorylation and dephosphorylation

Protein phosphorylation: Nature’s favorite way of decorating proteins

Click chemistry and its application to proteomics

Why Is Bovine Serum the Preferred Standard for Protein Assays?

How to Select the Best Protein Assay

DNFB-Sanger’s reagent for detection of free amino acids

How to Iodinate Proteins with Iodo-Gen®

Hot Start PCR: An Overview

Use of Chaperones in Recombinant Protein Expression

Using Western Transfer for Protein Detection: Some Commonly Used Methods

The Basics of Genomic DNA Extraction

Iodination of Proteins with Bolton-Hunter Reagent

Multiplex PCR, Benefits over traditional PCR

Best Methods to Stain Proteins After Protein Electrophoresis

Dyes, Ions, or Fluorescent Stains: What Are the Best Ways to Visualize Protein In Gels?

Best Ways to Remove Detergents in Protein Samples

Different Types of Extraction Buffers and When to Use Them

Preventing Carbamylation When Using Urea as a Protein Solubilization Buffer

8 Factors to Consider when Selecting a Protein Cross-linker

BCA or Bradford Protein Assay: Choosing Between the Two

Four Methods for Endosomal Isolation

Molecular Beacons: Dual Labeled Probes

Mycoplasma Contamination in Cell Cultures: Detection & Prevention

Antibody Selection for Immunohistochemistry

Protein Purification with IMAC

A Guide to Sample Preparation for Immunofluorescence

Detection of Apoptosis by TUNEL Assay

Luciferase Reporter Assays: An Overview

Protein Estimation Methods

Host Cell Protein Analysis: Detection in Biotherapeutics

Spotting Fragmentation Patterns When Using Mass Spectrometry

Tips for Preventing Protein Aggregation & Loss of Protein Solubility

How to set up a teaching biotech lab

How to Lyse Bacterial Cells

Why Start a High School Biotech Course?

A Quick Guide to Choosing Fluorescent Dyes for Protein Labeling

Biotech Education: Good for Students, Teachers & All

TOP 5 REASONS FOR A COLLEGE BIOTECH PROGRAM

Three most common ELISA Challenges

The Most Basic Biotech Lab Equipment, Materials & Necessities

Physical Disruption or Chemical Cell Lysis? How to Decide

Organic Solvent Extraction of Proteins

Specificity of Immunostaining methods: How to choose a control?

Sample preparation for Mass spectrometric analysis

Blue Native Polyacrylamide Gel Electrophoresis: A simple tool for characterization of Protein Complexes

Strategies to stabilize aggregate-prone proteins in E.coli

Tips for Selecting Carrier Proteins

Role of additives in cell lysis

A Quick Overview of Phenol Extraction

Microfluidic 3D cultures: An advanced tool to study the cultures at a single-cell level

Tris Tricine SDS PAGE: What is it and how to PERFORM it?

Contract Manufacturing Solutions for Biotech & Life Sciences

cGMP: What is it and what are the benefits?

Detergent Screening For Membrane Protein Extraction: What To Choose?

Ride the GeneCAPSULE & Explore Our Story!

Membrane Protein Extraction: The Basics

A Tutorial for 3D Cell Culture System: Bridging the Gap between in-vitro and in-vivo cultures

Molecular diagnostics- Lateral flow assay

A guide to in-vitro cultures: which matrix to be considered

Loop Mediated Isothermal Amplification (LAMP)

Genome-wide Ribosome footprint profiling

An Overview of Histology Fixatives

Why teach biotechnology in schools?

Methods of Determining Protein Stability

The Advantages of Using Trypsin for Mass Spectrometry

Antibody Labeling: Which Method Should You Use?

Protein Tags: How to Choose?

8 Techniques to Clone A Gene: Which Method is THe BEst FOr You?

Post Translational Modifications: what expression system to choose?

The Advantages of Biotinylation Tagging in Protein Purification

The Basics of Affinity Purification/Affinity Chromatography

Restriction Enzyme Analysis: How to Make the Cut

Proteomic Grade Detergents: Why you should use them

Exosome isolation by centrifugation, filtration, immunoaffinity & more

Multiplex Immunohistochemistry

Protein Solubility & Refolding Active Proteins from Inclusion Bodies

The Sandwich ELISA: Process and Practical Applications

How to Improve Spot Resolution During 2D-Electrophoresis

Recombinant Protein Expression Systems: Pros & Cons

Cold-Shock Keeps Recombinant Proteins out of Inclusion Bodies

BONCAT (Bioorthogonal Non-canonical Amino Acid Tagging): a method to define the translatome

Basics of Using Cytology for Cancer Testing

SILAC: A General Workflow for Improved Mass Spectrometry

SILAC for Improved Mass Spectrometry & Quantitative Proteomics

Key Differences of H&E and Special Stains for Immunohistochemistry

Study Protein to Protein Interaction with Protein Cross Linking to Glass

When to Use Immunohistochemistry (IHC) Detection vs. a Direct Method

Traction Force Microscopy for cell interaction with extracellular matrix

Immunoprecipitation Protocol: Critical Parameters

Immunoprecipitation Protocol: The Basics

MTT Assay for Cytotoxicity

Protein Storage For Protein Stability And Less Protein Degradation

Is Your BCA Protein Assay Really the Best Choice?

Remove BSA & Gelatin from Antibody Solutions for Antibody Labeling

Bacterial Growth Curves using a Spectrophotometer (Turbidimetric Determination)

Speed Up Protein Purification with FastPure™ Spin Columns

Overcome effects of detergents & reducing agents in protein estimation

Chromatography Series Part I: Liquid Chromatography Basics

How to isolate membrane receptor complexes from cells

How much biotin is coupled to my protein? The HABA/Avidin Assay

Sputum Processing, from viscous mucus to homogenous samples

Role of reporter genes to assay for transcription factors & more

High Efficiency & Stability Protein CrossLinking with EDC & NHS

Role of Non-Detergent Sulfobetaines in Protein Purification

Ammonium Sulfate Protein Precipitation: The key to Salting-Out

Modifying Oligonucleotide 5'-Phosphates By EDC for Improved Coupling

Optimize Elution Conditions in Affinity Chromatography to Antibodies

Immunoprecipitations (IP) for Protein Purification & Interactions Studies

How to select the Right protease inhibitor

Protect proteins with cryoprotectants & protein concentration

ELISA Blocking Agents & Blocking Solutions

Optimize Antigen and Antibody Concentration for Western Blots

Advantages of Magnetic Beads for Protein Immunoprecipitation

Detergents and their role in successful SDS-PAGE electrophoresis

Polyclonal Antibody Advantage for Antigen and Epitope Polymorphism

DNA Extraction: Difference in Plasmid and Genomic DNA Isolation

Mitochondrial DNA from Fungi (yeast) via Spheroplasts

CNBr-activated resin to immobilize ligands for affinity chromatography

Western Blot Blocking: Tips and Tricks for Blocking Agents

The Differences between a Protoplast and a Spheroplast (And Their Subsequent Uses)

Cellulase, a lytic enzyme to break plant cell walls for protein extraction

Stripping Membranes: A Great Addition to Western Blot Protocols

ECL (Enhanced Chemiluminescence) Reagents: Enhanced How?

Key Protein Concentration & Enrichment Techniques | G-Biosciences

Importance of detergent micelle levels in membrane protein purification

Epoxy-activated resin: a versatile affinity chromatography support

Plant Protein Extraction and Protein Precipitation Techniques

Specific plant tissue sample preparation & protein extraction methods

Cell Disruption Techniques: Sonication, Dounce Homogenizer & More

Non-Specific Binding: Why It Needs to be Blocked in Western blots!

Precautions with SDS Stock Solutions Preparation | G-Biosciences

Western Blot Blocking: Tips and Tricks

Cell Lysis: 5 Common Cell Disruption Methods | G-Biosciences

Detergents: Ionic, Non-Ionic, and Zwitterionic. What's the Difference?

Buffer Solution Preparation: An Essential Skill for Researchers

Plant vs Animal Cells: Break the Cell Wall with Lytic Enzymes

Western blot: Constant current or constant voltage; which is best?

Best Blocking Buffer Selection for ELISA & Western Blot

Polyclonal Antibody: Advantage over Monoclonals?

Viability Assays: Different Types and Their Use in Cell Death Research

What is Protein Electrophoresis and When Should It Be Used?

A Derivatizing Agent for Glutathione Assays

Glutathione Assays To Monitor Oxidative Stress

The Advantages of Loading Controls in Western Blotting

Tips for Preventing a High Background During Western Blotting

The Top Methods for DNA Denaturation

What is Drop Dialysis: An Overview

How to Ensure Proper Protein Transfer to a Membrane

Role of Lytic Enzymes in Protein and DNA Extraction

How to protect proteins during protein extraction

Coupling of ligands for carbohydrate affinity chromatography

Gel Filtration and Its Role in Desalting and Buffer Exchange

Fast Flow Agarose Resins: What's The Real Difference?

Applications of important lectins

How to Determine the Chemical Solubility of a Product

ELISA Substrates: A Selection Guide

Common Subcellular Markers You Need to Know About

How to extract biologically active proteins from the cells and tissue of organisms using detergent based lysis method

Role of Proteases in Mass Spec Identification of Proteins

Role of Adjuvants in Antibody Production

How to Check Western Transfer before using expensive antibodies

How to visualize proteins after electrophoresis

The 3 Types of Crosslinking Reagents and When to Use Them

Using Chaotropic Extraction Buffers to Preserve the Native Charge of Protein

Why Is Lactate Dehydrogenase (LDH) Release A Good Measure For Cytotoxicity?

Should I Use A Native Or Denaturing Gel?

Why Today's High School Biology Classes Are Teaching Biotech

Why Does Tyrosine and Tryptophan Have Effect in Protein Determination and to What Degree?

How to Safely Use Detergents during Protein Extraction

Tips for Preparing Phosphate Buffers

Coupling to Carrier Proteins: An Overview

Selecting Protein Assays? These Are the Top Factors That Can Impact Accuracy

Why is Trypsin the Preferred Choice for Peptide Generation?

Why Should I Have aN Inhibitor Cocktail?

How To Determine Degree of Protein Labeling

How To Elute Tightly Bound Glyoproteins from Concanavalin A (Con A) Agarose

Monoclonal vs Polyclonal Antibodies

Recombinant Antibodies: An Overview

How to determine reactivity of NHS esters on biotinylation and cross-linking reagents.

How to Stain Glycoproteins in Polyacrylamide (PAGE) Gels

Hands-on Science as a Teaching Advantage

Why do I need a protease inhibitor?

Using Protease Assays for Accurate Protease Detection

The Common Failures That can Occur When Using Dialysis Bags

3 Protein Gel Stains and When You Should Use Them

Why BCA Protein Assay is Overhyped

Are Total Protein Membrane Stains Compatible With IR Imaging Systems?

What's best? Housekeeping genes or total protein for Western blot loading controls

Enhanced Chemiluminescence: What is it?

How to Deal with Interfering Agents in Protein Estimation Assays

Coomassie, Enhanced Coomassie, and Colloidal Coomassie: What’s the Difference?

How to Choose between G250 or R250 Coomassie Dyes

Dialysis - Knot the Way to Do It

Why SDS is a Necessary Evil in PAGE

Colorimetric or Copper Based Protein Assays - How to Decide Which is Best

Why do PVDF membranes require a methanol soak?

Which Stain is Most Compatible with Mass Spectrometry?

How to Tell if You Should Use Gel Filtration or Dialysis

Chromogenic Protein Detection: Is It a Suitable Alternative to Chemiluminescence?

How to Improve Dialysis Efficiency

The Basics of Hydrophobic Interaction Chromatography

Membrane Protein Isolation and Purification With Phase Separation

Magnetic Beads for Immunoprecipitation

PVDF or Nitrocellulose - Which Membrane is Best?

A Look into the 6XHis Tag and its Uses

Which agarose (sepharose) to choose? 2, 4 or 6%? Crosslinked?

Spectrophotometry and Its Application in Protein Estimation

Using Tags in Protein Purification: When Should You Use Them?

How Proteins Interact with DNA and RNA to Influence Nucleic Acid

What You Need to Know About NTA and IDA Ligands

A Guide to Protein Cross Linkers

Interfering Agents of 2D Electrophoresis and How to Remove Them

Special Considerations When Extracting Plant Proteins

8 Protein Tags Explained

Dialysis in Protein Research: Understanding the Basics

How to clean the sinter of a Büchner flask?

Biological Buffers: pH Range and How to Prepare Them

Determining Protein Molecular Weight with SDS-PAGE: An Overview of the Process

Protein Analysis and Identification by Mass Spectrometry: Taking a Closer Look

Protein Extraction and Isolation Accessories for Sample Preparation

Uncovering the Importance of Detergents in the Study of Membrane Proteins

Solubilized Proteins: Understanding Their Importance

Sample Preparation for Western Transfer: A Definitive Guide

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)

What Assay Development Accessories Should You Use For Protein Binding? (Part 3 f 3)

What Assay Development Accessories Should You Use For Protein Binding? (Part 2 f 3)

What Assay Development Accessories Should You Use For Protein Binding? (Part 1 f 3)

What Are the Most Commonly Used Protein Estimation Assays? (Part 2 of 2)

What Are the Most Commonly Used Protein Estimation Assays? (Part 1 of 2)

The Top 8 Applications for Protein Cross-Linkers and Why You Need Them (Part 2)

The Top 8 Applications for Protein Cross-Linkers and Why You Need Them (Part 1)

What Are the Detergents Commonly Used in Proteomics?

Protease Inhibitors and Cocktails: Getting Down to the Basics

Understanding How 2D Protein Electrophoresis Works: A Step-by-Step Process

How to generate an antibody affinity column for protein purification?

Genomic DNA Extraction Protocol: Isolating Your Genomic DNA from Your Sample (Part 2 of 2)

Genomic DNA Extraction Protocol: Isolating Your Genomic DNA from Your Sample (Part 1 of 2)

Creating Living Factories for Recombinant Proteins (Part 2 of 2)

Creating Living Factories for Recombinant Proteins (Part 1 of 2)

Preparing Phosphate Buffers: How to Do It Right

Detecting Properties of Protein Using Protein Binding Assay (Part 3 of 3)

Detecting Properties of Protein Using Protein Binding Assay (Part 2 of 3)

Detecting Properties of Protein Using Protein Binding Assay (Part 1 of 3)

Protein Fractionation: Techniques for Isolating Proteins of Interest

Understanding the Protein Electrophoresis Workflow (Part 3)

Understanding the Protein Electrophoresis Workflow (Part 2)

Understanding the Protein Electrophoresis Workflow (Part 1)

Using Carrier Proteins for Successful Antibody Production

Protein Labelling 101: Understanding the Basics

Protein Lysis Buffer 101 – Components and Functions (Part 2)

Protein Lysis Buffer 101 –Components and Functions (Part 1)

How to Reduce Pipetting Errors When Working with Apoptosis Assays

DNA Purification: Which Solvent Should You Use?

Using Ampicillin in Plasmid DNA Isolation: What You Need to Know

Protein Immunoprecipitations: Which method to use?

DNA Purification vs. RNA Purification: What is the Difference?

A More In-Depth Look at the Classification of Cytotoxicity Assays

Assay Development 101: A Beginner’s Guide

Understanding the Role of Proteases in DNA Extraction

Sepharose or Agarose for protein resins, which should I choose?

Sample Clean Up: Everything You Need to Know

Using Assays in Determining Protein Concentration

Commonly Used Apoptosis Assays in Apoptosis Research

Protein Fractionation in Protein Research: What Does It Do?

Protein Extraction: Why Is It More Difficult than DNA Extraction?

Uncovering the Role of Detergents in Protein Solubilization

DNA Purification: Why Should You Purify Your DNA Samples?

Polymerase Chain Reaction: What Importance Does It Hold?

Stock Solutions 101: Everything You Need to Know

Choosing Affinity Tags for Recombinant Protein Purification

Plasmid DNA Isolation through Alkaline Lysis: How Does It Work?

Understanding Protein Electrophoresis: Techniques and Applications

Using Protease Assays for Accurate Protease Detection

Reagents for Plasmid Purification and Colony Screening

Understanding DNA Methylation and its Role in Gene Expression

Non-Radioactive Assays for Cell Toxicity and Cell Proliferation Determination

Chromatographic Methods for Protein Purification

Identification of Protein Using Western Blot Reagents

What is Cell Cytotoxicity and How to Measure It?

Bradford Protein Assay: Calculation of An Unknown Standard

What is the Difference Between Cell Death and Apoptosis?

What Protein Precipitation Techniques Are Used for Concentration and Clean Up?

What Assays are used in Protein Concentration Determination?

How Mass Spectrometry Helps in Protein Identification?

What is Protein Cross-Linking and Which Reagents are Used in it?

What are Protease Inhibitors and How Do They Work?

What is the Best Blocking Agent for Western Transfer?

Protein Separation by SDS-PAGE (PolyAcrylamide Gel Electrophoresis)

Techniques of Protein Extraction Through Cell Lysis

Work of Salt, Isopropanol and Ethanol in DNA Extraction

Using Polymerase Chain Reaction to Identify Different Strains of an Organism

4 Simple Steps To Prevent Genomic DNA Samples from Degrading Quickly

What is Protein Purification and What Techniques are Used During This Process?

What is the Role of Buffer System in Protein Extraction and Clarification?

How is Protein Electrophoresis Different from Western Blotting?

How to Calculate Protein Concentration Using The Bradford Assay

What is the Role of the Isoelectric Point of a Protein in its Purification?

14 Must Have Life Science Handbooks

What Protein Assay is Good for Estimating Peptides?

Go Green and Eliminate the Carcinogens from DNA & RNA Analysis

7 Common Problems with Western transfer Solved

The Secrets of Coupling with Biotin!

Biotin Labeling: Key Points to Selecting Your Biotin Agent

Seven Quick Solutions to Protein Electrophoresis Loading Problems

Protein Gel Staining: Detecting Small Peptides

Protein Concentration: Alternative Methods to UltraFiltration

Are Detergents Harmful To Your Protein?

Fractionation of Proteins: The Key To Protein Identification

How to isolate membrane proteins for 2D protein electrophoresis?

Protein Estimation Assays: Why Is There No Single Solution?

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium