The Protein Man's Blog | A Discussion of Protein Research

Affinity Purification Resins and Methods

Posted by The Protein Man on Oct 25, 2022 11:30:00 AM

Affinity purification (also called affinity chromatography) is recognized as the most powerful method of purification chromatography or enriching a protein of interest from a complex mixture such as a crude cell lysate, cell culture supernatant, or other samples. 

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What Are Monoclonal Antibodies, and How Are They Made?

Posted by The Protein Man on Oct 11, 2022 11:30:00 AM

Monoclonal antibodies (mAbs or MoAbs) are immune system proteins that were discovered in 1975 as a highly specific targeted molecule - it will bind to its target molecules with high specificity. It could take a load of drug or agent and deliver it to the target. Because of its specificity, monoclonal antibodies have found its use in medical science to treat a wide variety of diseases, including some types of cancer, cardiovascular disease, autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, Crohn’s disease, and psoriasis, and minimize or eliminate the risk of transplant rejection. 

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Topics: Antibody Production

Epigenetics and Personalized Medicine

Posted by The Protein Man on Sep 27, 2022 11:15:00 AM

What is epigenetics and what roles does it play in disease prevention, treatment, and management? Can it be the key to effective personalized medicine? 

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Topics: DNA Extraction

ELISA Principles: Explained

Posted by The Protein Man on Sep 13, 2022 11:15:00 AM

What is ELISA?

ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique designed to detect and quantify target molecules such as peptides, proteins, antibodies, and hormones in biological samples. Like other types of immunoassays, ELISA relies on specific antigen-antibody interactions to detect a target antigen.

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Topics: Assay Development (ELISA)

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