The Protein Man's Blog | A Discussion of Protein Research

Western Blotting: Stripping and Re-probing – Saving Your Time & Resources

Posted by The Protein Man on Apr 13, 2022 11:53:07 AM

What is a Western blot?

Western blotting is the process of transferring proteins from a gel onto a membrane to identify proteins of interest. The membrane is treated with antibodies that bind epitopes on the protein of interest. After further treatment with a secondary antibody, specific proteins in the sample can be visualized using detection techniques such as the Enhanced Chemiluminescence (ECL) method.

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Topics: Western Blotting

Different Types of 3D Cell Cultures

Posted by The Protein Man on Feb 15, 2022 12:10:36 PM

While two-dimensional (2D) cell cultures are still being used for basic research and drug discovery, screening, and testing, the use of 3D cell cultures is fast gaining popularity since it effectively addresses many of the limitations commonly encountered in 2D cell cultures. Specifically, three-dimensional (3D) cell cultures:

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DNA and mRNA Vaccines: A Side-by-Side Comparison

Posted by The Protein Man on Nov 2, 2021 2:00:00 PM

What’s the difference between DNA and mRNA vaccines and how do they work? Are they better than traditional vaccines? With all the exciting life-saving possibilities these modern technologies offer, it’s time to take a closer look.

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Topics: Molecular Biology

Telomeres, Telomerase and Cancer

Posted by The Protein Man on Aug 24, 2021 2:00:00 PM

Telomeres and telomerase may play a crucial role in cellular aging, cancer initiation, and tumor survival, but a thorough understanding of the mechanisms involved in telomere length (TL) maintenance and telomerase expression gives experts the tools they need to develop diagnostic tools, determine disease prognosis, and formulate effective anticancer therapeutics.

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Topics: Protein Estimation

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