The Protein Man's Blog | A Discussion of Protein Research

DNA Extraction: Difference in Plasmid and Genomic DNA Isolation

Posted by Protein Man on Jun 20, 2017 2:30:00 AM

Isolating genomic and plasmid DNA for further investigation and downstream application (e.g. PCR, sequencing, etc.) requires totally different protocols. While isolating genomic DNA merely requires you to crack open the cell walls and purify the resulting sample, extracting plasmid DNA may be a bit trickier and more complicated than this. Here’s a rundown on how these techniques differ.

Read More

Topics: Molecular Biology

Mitochondrial DNA from Fungi (yeast) via Spheroplasts

Posted by Protein Man on Jun 13, 2017 2:30:00 PM

INTRODUCTION

When we speak of mitochondria, anyone with knowledge of life science could tell you of its presence in animal cells and absence in plant cells. However, it should not be forgotten that another kingdom shares this cellular powerhouse - kingdom fungi.

Read More

Topics: Molecular Biology, Protein Extraction

CNBr-activated resin to immobilize ligands for affinity chromatography

Posted by Protein Man on Jun 6, 2017 2:30:00 PM

Commercially available affinity purification supports are designed on the principle of specific surface interactions among biomolecules, including, but not limiting to, antigen-antibody, enzyme-ligand and lectin-carbohydate. Affinity chromatography is one of the most efficient tools used for purification of biomolecules of interest, including proteins, glycoproteins, lipids and nucleic acids. In affinity chromatography, one of the interacting molecules is covalently bound to the resin and is addressed as a ligand. The stationary ligand bound resin interacts with the ligand interacting proteins or biomolecules, which are passed through the resin in mobile phase and hold them to the resin. These molecules are later eluted as a purified fraction with an elution buffer.

Read More

Topics: Protein Purification

Get the latest from The Protein Man

Protein_Purificaiton
Ellyn Daugherty's Biotechnology: Science for the New Millennium