The Protein Man's Blog | A Discussion of Protein Research

How Chaperone-Assisted Protein Folding Works

Posted by The Protein Man on Sep 23, 2020 1:00:00 AM

How Chaperone-Assisted Protein Folding Works

What are chaperone-assisted proteins and what roles do they play in protein folding? Basically, chaperones are a group of proteins essential for promoting cell viability and stability. Thus, they are also responsible for ensuring that various processes such as translocation, degradation, and folding are carried out properly at all times.

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The Amazing Origins of Antibody Diversity

Posted by The Protein Man on Sep 15, 2020 3:00:00 PM

The Origins of Antibody Diversity

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Topics: Molecular Biology, Western Blotting, Assay Development (ELISA)

How to Prepare Samples for Western Blot Analysis

Posted by The Protein Man on Sep 8, 2020 9:00:00 AM

What is Western Blot Analysis?

Western blotting or immunoblotting is an indispensable technique, almost every published paper in area of molecular cell biology uses western blotting for detecting specific proteins in a sample of tissue homogenate or cell lysates. Western blotting combines resolving power of polyacrylamide gel electrophoresis (PAGE) or SDS-PAGE and specificity of antibodies to detect target proteins.  Proteins are resolved on the basis of their molecular weight in SDS-PAGE and transferred from the polyacrylamide gel onto the membranes (Nitrocellulose or PVDF), which creates an exact replica of the protein separation pattern on the membrane. After transferring the proteins to the membrane, the membrane needs ‘blocking’ to ‘block’ non-specific binding sites on the surface of the membrane. Blocking is usually performed with Bovine Serum Albumin, Skimmed milk or purified milk Casein.

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The era of messenger RNA vaccines

Posted by The Protein Man on Aug 28, 2020 10:00:00 AM

“Trumping the vaccine game”

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