The Protein Man's Blog | A Discussion of Protein Research

14 Must Have Life Science Handbooks

Posted by The Protein Man on May 4, 2012 1:18:00 PM

Click the covers to download the Handbooks

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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers

Protein Concentration: Alternative Methods to UltraFiltration

Posted by The Protein Man on Jan 25, 2012 11:48:00 AM

Question

I am needing a product to concentrate 20mg of protein. My current volume is 15mL and I need to get to under 5mL volume. I have used spin columns in the past and my protein has gotten stuck on the cellulose membrane, so I am looking for alternatives.

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Topics: Sample Clean Up, Protein Concentration

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