The Protein Man's Blog | A Discussion of Protein Research

Tips for Selecting Carrier Proteins

Posted by The Protein Man on Nov 22, 2018 2:30:00 PM

As you are no doubt aware, carrier proteins play a significant role in antibody production. While immunogenic peptides are generally considered to be good epitopes by themselves, they are too small and lack the additional motifs required to link with class II molecules and T-cell receptors. Thus, they do not have the ability to boost the antibody response.

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Topics: Antibody Production

Antibody Labeling: Which Method Should You Use?

Posted by The Protein Man on Sep 4, 2018 2:30:00 PM

When it comes to labeling your antibodies for downstream application requiring signal detection, you have two choices. You can either go for the direct approach or the indirect approach. How do you know which method to use? Here are some things you need to know to make sure you pick the right method for the intended application.

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Topics: Antibody Production, Protein Labeling

Remove BSA & Gelatin from Antibody Solutions for Antibody Labeling

Posted by The Protein Man on Jan 9, 2018 2:30:00 PM

Purified and commercial antibodies are routinely stored in buffers that contain the proteins bovine serum albumin (BSA) and gelatin as these act as stabilizers during long term storage.  The presence of 0.2-1% (2-10mg/ml) BSA and/or gelatin help to stabilize antibody solutions that are less than 1mg/ml during long term storage.  For the majority of immunodetection techniques (ELISA, Western blotting, immunoprecipitation) the presence of the stabilizers do not interfere with these immunodetection techniques and can be used without clean up.  

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Topics: Antibody Production

Polyclonal Antibody Advantage for Antigen and Epitope Polymorphism

Posted by The Protein Man on Jun 27, 2017 2:30:00 PM

Monoclonal antibodies are often marketed as being superior to polyclonal antibodies. While this might be the case in many applications, this is not necessarily always true. In order to understand what type of antibody is best suited for a particular application, one needs to understand how they are generated.

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Topics: Antibody Production

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