This guide applies to many, but not all antibodies. Always consult the product insert of the antibody for storage and handling instructions.
The availability of purified antibodies is essential to various applications. While there are various methods of purification, affinity chromatography is the most popular antibody purification technique.
As you are no doubt aware, carrier proteins play a significant role in antibody production. While immunogenic peptides are generally considered to be good epitopes by themselves, they are too small and lack the additional motifs required to link with class II molecules and T-cell receptors. Thus, they do not have the ability to boost the antibody response.
Topics: Antibody Production
When it comes to labeling your antibodies for downstream application requiring signal detection, you have two choices. You can either go for the direct approach or the indirect approach. How do you know which method to use? Here are some things you need to know to make sure you pick the right method for the intended application.