The Protein Man's Blog | A Discussion of Protein Research

Antibody Basics Revisited (Do’s and Don’ts of Antibody Handling)

Posted by The Protein Man on Jun 16, 2020 1:00:00 PM

This guide applies to many, but not all antibodies.  Always consult the product insert of the antibody for storage and handling instructions.

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Topics: Western Blotting, Sample Clean Up, Antibody Production

Antibody Purification by Affinity Chromatography

Posted by The Protein Man on Apr 28, 2020 2:30:00 PM

The availability of purified antibodies is essential to various applications. While there are various methods of purification, affinity chromatography is the most popular antibody purification technique.

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Topics: Protein Purification, Antibody Production

Tips for Selecting Carrier Proteins

Posted by The Protein Man on Nov 22, 2018 2:30:00 PM

As you are no doubt aware, carrier proteins play a significant role in antibody production. While immunogenic peptides are generally considered to be good epitopes by themselves, they are too small and lack the additional motifs required to link with class II molecules and T-cell receptors. Thus, they do not have the ability to boost the antibody response.

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Topics: Antibody Production

Antibody Labeling: Which Method Should You Use?

Posted by The Protein Man on Sep 4, 2018 2:30:00 PM

When it comes to labeling your antibodies for downstream application requiring signal detection, you have two choices. You can either go for the direct approach or the indirect approach. How do you know which method to use? Here are some things you need to know to make sure you pick the right method for the intended application.

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Topics: Antibody Production, Protein Labeling

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