The Protein Man's Blog | A Discussion of Protein Research

What is the Link between Protein Folding and Prion Disease?

Posted by The Protein Man on Aug 12, 2020 5:00:00 PM

Prion diseases (also referred to as transmissible spongiform encephalopathies, or TSEs) are a group of progressive, untreatable, and fatal neurodegenerative conditions affecting both humans and animals. Generally, these conditions are characterized by long incubation periods (usually between 5 to 20 years), disruption in the normal tissue structure (resulting in holes and vacuole formation in the neurons), and the absence of an inflammatory response.

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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Cytotoxicity Assays, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Buffers & Chemicals

How the cilium became a ‘villain’ in Apicomplexan parasites?

Posted by The Protein Man on Jul 15, 2020 1:45:00 PM

Evolution of ancient cellular components paves a path to parasitic adaptations in Apicomplexan parasites.

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Topics: Molecular Biology, Protein Electrophoresis, Protein Concentration, Protease Inhibitors, Protein Labeling

Exposure to Intestinal microbes in early life can influence development of thymic lymphocytes : A review of current literature

Posted by The Protein Man on Jun 23, 2020 10:11:22 AM


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Topics: Western Blotting, Protein Electrophoresis, Protein Estimation, Protein Concentration, Protease Inhibitors, Protein Fractionation, Protein Labeling

Best Methods to Stain Proteins After Protein Electrophoresis

Posted by The Protein Man on Jun 18, 2019 2:30:00 PM

Protein samples are analyzed by denaturing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total protein lysates or protein samples from purification experiments are separated on 1D and 2D gels depending on their molecular weights and PI.  Appropriate staining techniques are used to detect proteins on SDS-PAGE gels depending on the protein of interest, downstream experiments like Western blotting, mass spec analysis etc.,

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Topics: Protein Electrophoresis

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