Prion diseases (also referred to as transmissible spongiform encephalopathies, or TSEs) are a group of progressive, untreatable, and fatal neurodegenerative conditions affecting both humans and animals. Generally, these conditions are characterized by long incubation periods (usually between 5 to 20 years), disruption in the normal tissue structure (resulting in holes and vacuole formation in the neurons), and the absence of an inflammatory response.
Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Cytotoxicity Assays, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Buffers & Chemicals
Evolution of ancient cellular components paves a path to parasitic adaptations in Apicomplexan parasites.
Protein samples are analyzed by denaturing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total protein lysates or protein samples from purification experiments are separated on 1D and 2D gels depending on their molecular weights and PI. Appropriate staining techniques are used to detect proteins on SDS-PAGE gels depending on the protein of interest, downstream experiments like Western blotting, mass spec analysis etc.,
Topics: Protein Electrophoresis