The Protein Man's Blog | A Discussion of Protein Research

Four Methods for Endosomal Isolation

Posted by The Protein Man on Apr 16, 2019 2:30:00 PM

As a functional unit of a living entity, cells are very complicated and consist of exceptionally high number of biomolecules with completely different physical and chemical properties. The complexity of the biomolecules profile is even more intricately woven depending upon their subcellular location. With the limited resolution of the separation and localization technologies, it is imperative to employ fractionation methods for protein profiling and expression analysis.  Even the lesser abundant proteins can be analysed if screened after prefractionation. Sensitive and accurately fractionated samples help in convolution of methods such as mass spectrometry or in-vitro analysis for the detection of proteins at the level of organelles or transportation compartments.

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Topics: Protein Fractionation

Key Protein Concentration & Enrichment Techniques | G-Biosciences

Posted by The Protein Man on Apr 18, 2017 2:30:00 PM

What is protein enrichment and why is there a need for it? Basically, protein enrichment is a technique where proteins of interest in a biological sample are concentrated to make them more suitable for identification and downstream analysis.

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Topics: Protein Concentration, Protein Fractionation, Protein Extraction

Protein Fractionation: Techniques for Isolating Proteins of Interest

Posted by The Protein Man on Aug 8, 2013 5:00:00 AM

Question:

What are the common techniques used in protein fractionation?   

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Topics: Protein Fractionation

Protein Fractionation in Protein Research: What Does It Do?

Posted by The Protein Man on Mar 20, 2013 5:00:00 AM

Question:

What is the role of protein fractionation in protein research?  

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Topics: Protein Fractionation

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