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The Protein Man

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Types and Grades of Water Used in Research and Industrial Application

Posted by The Protein Man on Jul 29, 2022 8:45:00 AM

What Are the Different Types and Grades of Water Used in Research and Industrial Application?

Water grade is dependent on the nature of impurities one is considering. Water impurities are generally minerals, chemicals and organic, and biological. Average city water will contain all these impurities in different proportions. City water supplies and their level of acceptable impurities is highly dependent on where one lives and the nature of local rules and regulations.

Depending on the nature of water use, commonly used terminologies for water grades are molecular grade water, DNase, and RNase, and nuclease free water, DEPC treated water, proteomic grade water, endotoxin free water, distilled water, deionized water, water for injection (WFI), pharma grade water, biotech grade water, biological grade water, cell culture grade water, HLPC grade water, Ultapure grade water.

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Topics: Water

Biocides And Their Many Applications

Posted by The Protein Man on Jun 20, 2022 11:30:00 AM

What Are Biocides?

Biocides are non-antibiotic agents used as preservatives, disinfectants, or antiseptics. Although most biocides are chemical agents, microorganisms can also be used as biocides to suppress the growth of other organisms. Biocides act either by killing microorganisms or inhibiting their growth. Biocides can be further classified by their intended application. Preservatives are added to products to increase their shelf-life by preventing microbial decomposition. Disinfectants are agents that are applied to inanimate objects and surfaces, whereas antiseptics are agents applied to living tissue such as mucous membrane or skin. In today’s world, biocides are frequently encountered.  Preservatives are ubiquitous and are found in a diverse array of products ranging from food, beverages, and cosmetics to pharmaceutical drugs, biological samples, and research products. Likewise, the use of disinfectants has increased dramatically in response to the worldwide pandemic, and antiseptics are increasingly important in hospital settings.

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Topics: Molecular Biology

Western Blotting: Stripping and Re-probing – Saving Your Time & Resources

Posted by The Protein Man on Apr 13, 2022 11:53:07 AM

What is a Western blot?

Western blotting is the process of transferring proteins from a gel onto a membrane to identify proteins of interest. The membrane is treated with antibodies that bind epitopes on the protein of interest. After further treatment with a secondary antibody, specific proteins in the sample can be visualized using detection techniques such as the Enhanced Chemiluminescence (ECL) method.

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Topics: Western Blotting

Different Types of 3D Cell Cultures

Posted by The Protein Man on Feb 15, 2022 12:10:36 PM

While two-dimensional (2D) cell cultures are still being used for basic research and drug discovery, screening, and testing, the use of 3D cell cultures is fast gaining popularity since it effectively addresses many of the limitations commonly encountered in 2D cell cultures. Specifically, three-dimensional (3D) cell cultures:

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