The Protein Man's Blog | A Discussion of Protein Research

The Protein Man

The Protein Man

Recent Posts

Subcellular Fractionation: ‘Exploring cells through centrifugation'

Posted by The Protein Man on Jun 30, 2020 1:47:00 PM



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Topics: Protein Purification

Exposure to Intestinal microbes in early life can influence development of thymic lymphocytes : A review of current literature

Posted by The Protein Man on Jun 23, 2020 10:11:22 AM


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Topics: Western Blotting, Protein Electrophoresis, Protein Estimation, Protein Concentration, Protease Inhibitors, Protein Fractionation, Protein Labeling

Antibody Basics Revisited (Do’s and Don’ts of Antibody Handling)

Posted by The Protein Man on Jun 16, 2020 1:00:00 PM

This guide applies to many, but not all antibodies.  Always consult the product insert of the antibody for storage and handling instructions.

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Topics: Western Blotting, Sample Clean Up, Antibody Production

The Use of Spectral Scanning in Nucleic Acid Purity Assessment

Posted by The Protein Man on Jun 9, 2020 12:28:00 PM


Spectrophotometric analysis, also called spectral scanning, of biomolecules serves two main purposes – the quantitation of nucleic acids (DNA and RNA) and purity assessment. Since the amount or concentration and purity of the DNA or RNA in a solution significantly affect the results of subsequent downstream applications, establishing these values early on can reduce, if not totally eliminate, the risk of committing errors and guarantee optimum results.

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Topics: Molecular Biology, Apoptosis Assays, Assay Development (ELISA), Detergents, Buffers & Chemicals, Cytology & Histology

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