Gene expression is the fundamental process by which the genetic code within DNA is translated into functional proteins, the workhorses of the cell. This intricate process involves two key steps: transcription (where DNA is transcribed into RNA) and translation (where RNA is translated into protein). Understanding the journey from RNA to protein is not only a cornerstone of molecular biology but also a critical area of research for advancements in medicine, biotechnology, and synthetic biology.
Topics: Translation, Taq DNA Polymerase, quantitative Polymerase Chain Reaction (qPCR), Gene expression, Transcription, complementary DNA (cDNA), Reverse Transcriptase (RT)
Size Exclusion Chromatography (SEC) remains a cornerstone method for protein purification, valued for its ability to deliver high-purity samples with minimal loss of biological activity. From structural analysis to functional studies and therapeutic development, the purity of protein preparations directly impacts experimental outcomes.
Topics: Protein Purification, Size exclusion chromatography,, Chromatographic resins, Gel filtration chromatography
Magnetic bead technology has emerged as a linchpin, offering researchers a robust and flexible
tool for a wide range of applications—from isolating specific cell populations to streamlining
immunoprecipitation protocols. Magnetic beads are used to immobilize molecules (e.g., proteins,
enzymes, peptides, antibodies, nucleic acids) on a solid phase, thereby separating them from the
lysate. When the sample is added to the beads and a magnetic field is applied to the mixture, the
target molecule binds to the beads, allowing it to be separated from the rest. The beads are then
washed to remove impurities, and the target molecule is eluted using an appropriate buffer.
Topics: Protein Purification, Cell separation, Nucleic acid purification, Immunoprecipitation, Next-generation sequencing (NGS), PCR/qPCR
