The Protein Man's Blog | A Discussion of Protein Research

Sputum Processing, from viscous mucus to homogenous samples

Posted by The Protein Man on Oct 10, 2017 2:32:00 PM

Sputum Liquefaction: from viscous, heterogeneous mucus to equivalent, homogenous samples

Sputum processing has one main purpose; to render the viscous, heterogeneous specimen to a homogenous state (evenly distributed suspension). This homogenous suspension will be dispensed into multiple aliquots for cryopreservation, smear microscopy, gram stain, and culture. Each aliquot should be representative of the original specimen. This can be accomplished through the process of sputum liquefaction.

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Topics: Sample Clean Up

Plant Protein Extraction and Protein Precipitation Techniques

Posted by The Protein Man on Mar 28, 2017 3:00:00 PM

Extracting proteins from plant and animal sources are two different things. While animal proteins can easily be extracted, working with plant proteins can be quite a challenge. Why? Plant proteins are more difficult to extract since they are protected by a rigid cell wall that contains several interfering agents (i.e proteases).

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Topics: Sample Clean Up, Protein Extraction

Dialysis - Knot the Way to Do It

Posted by The Protein Man on Apr 17, 2015 10:00:00 AM

Dialysis_-_Knot_the_Way_to_Do_ItThere are certain things that you should not do when performing dialysis in the laboratory. To ensure that all unwanted small molecular weight substances (such as reducing agents, non-reacted crosslinking or labeling reagents, and preservatives) are removed and facilitate a more efficient buffer exchange for macromolecular samples such as proteins, here are some of the things you need to avoid at all cost.   

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Topics: Sample Clean Up

How to Tell if You Should Use Gel Filtration or Dialysis

Posted by The Protein Man on Feb 27, 2015 10:00:00 AM

largeBasically, there are two methods that are commonly used in the preparation of protein samples to make them suitable for long-term storage and compatible with downstream applications - dialysis and gel filtration. So, how do you know which one to use? Here are some things you need to consider if you want to increase your chances of making the right decision.

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Topics: Sample Clean Up

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