The Protein Man's Blog | A Discussion of Protein Research

Which agarose (sepharose) to choose? 2, 4 or 6%? Crosslinked?

Posted by The Protein Man on Oct 15, 2014 9:00:00 AM

Agarose beads are small spherical beads of agarose gel which are commonly used in gel filtration or molecular size exclusion chromatography and biomolecular purification and immobilization. These beads act as porous gel to filter mixtures of molecules based on their individual sizes. And since these beads are easy to activate, they can also be used to bind biomolecules in a reversible or irreversible manner. In addition, their inert nature and unique internal surface area can also be activated for ligand attachment, making them the ideal basis for various affinity chromatography beads such as protein A and G, and glutathione.

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Topics: Protein Purification, Sample Clean Up

Solubilized Proteins: Understanding Their Importance

Posted by The Protein Man on Mar 5, 2014 5:00:00 AM

Question:

What are some important applications of solubilized proteins?  

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Topics: Sample Clean Up

Sample Clean Up: Everything You Need to Know

Posted by The Protein Man on Apr 10, 2013 5:00:00 AM

Question:

Sample Clean Up: Why You Should Do It?  

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Topics: Sample Clean Up

What Protein Precipitation Techniques Are Used for Concentration and Clean Up?

Posted by The Protein Man on Nov 3, 2012 1:15:00 AM

Question:

What precipitation techniques are used for concentration and clean up?  

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Topics: Sample Clean Up, Protein Concentration

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