Agarose beads are small spherical beads of agarose gel which are commonly used in gel filtration or molecular size exclusion chromatography and biomolecular purification and immobilization. These beads act as porous gel to filter mixtures of molecules based on their individual sizes. And since these beads are easy to activate, they can also be used to bind biomolecules in a reversible or irreversible manner. In addition, their inert nature and unique internal surface area can also be activated for ligand attachment, making them the ideal basis for various affinity chromatography beads such as protein A and G, and glutathione.
Which agarose (sepharose) to choose? 2, 4 or 6%? Crosslinked?
Posted by
The Protein Man on Oct 15, 2014 9:00:00 AM
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Topics: Protein Purification, Sample Clean Up
Solubilized Proteins: Understanding Their Importance
Posted by
The Protein Man on Mar 5, 2014 5:00:00 AM
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Topics: Sample Clean Up
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Topics: Sample Clean Up
What Protein Precipitation Techniques Are Used for Concentration and Clean Up?
Posted by
The Protein Man on Nov 3, 2012 1:15:00 AM
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Topics: Sample Clean Up, Protein Concentration