The Protein Man's Blog | A Discussion of Protein Research

Revisiting Eukaryotic RNA Processing

Posted by The Protein Man on Apr 20, 2021 9:15:03 AM

In eukaryotic cells, RNA processing occurs in the nucleus and the functional mRNA is transported to the cytoplasm. As soon as the mRNA transcript is synthesized from RNA polymerase II, RNA binding proteins (RBPs) attach to the mRNA transcript to prevent it from degradation during processing and exporting it from the nucleus. RBPs play an important role in RNA stability, maturation, transport, and localization. This process results in the formation of stable mature mRNA (lasts for several hours) than that in prokaryotes (5 seconds). 

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Topics: Molecular Biology

All about Proteinase K

Posted by The Protein Man on Mar 16, 2021 1:00:00 PM

Proteinase K is a serine protease, the presence of a catalytic triad characterizes serine proteases, the catalytic triad is a cluster of three amino acids that make the catalytic center and consists of serine, aspartic acid, and histidine amino acids, which can often vary but all of these enzymes have a nucleophile serine and the same catalytic mechanism. Proteinase K has a catalytic triad consisting of Ser 224, His 69, and Asp 39. The substrate recognition sites are made up of peptide chains, 99-104 and 132-136.

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Topics: Protein Purification, Molecular Biology

The Importance of BACs in Genome Sequencing (and Why They've Replaced YACs)

Posted by The Protein Man on Mar 11, 2021 2:00:00 PM

 

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Topics: Molecular Biology, Bioassays

ApoE4 and Alzheimer's Disease

Posted by The Protein Man on Mar 2, 2021 1:00:00 PM

Alzheimer’s disease (AD) is a progressive neurologic disorder that leads to the death of brain cells. People with this condition usually exhibit mild memory loss, impaired judgment, and vision (spatial) issues during the initial stages, but the symptoms gradually worsen over time.

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Topics: Molecular Biology, Cytotoxicity Assays, Bioassays

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