The Protein Man's Blog | A Discussion of Protein Research

14 Must Have Life Science Handbooks

Posted by The Protein Man on May 4, 2012 1:18:00 PM
The Protein Man

Click the covers to download the Handbooks

Protein Assay Handbook

  • Protein estimation assays
  • Apoptosis Assays
  • Cytotoxicity Assays
  • SAM Methyltransferase Assays
  • Protease Assays
  • Phosphatase Assays
  • Peroxide Assays
Protein Assay Handbook

Sample Preparation Handbook

  • Lysis Buffers & Systems
  • Protein Fractionation Kits
  • Dialysis (Micro) Systems
  • Electrophoresis Clean Up
  • Concentration Systems
  • Contamination Removal
Sample Preparation Handbook

Proteases & Phosphatases: Inhibitors, Enzymes & Assays

  • Protease Inhibitor Cocktails
  • Individual Protease Inhibitors
  • Protease Assays
  • Proteases for Mass Spectrometry
  • Sequencing Grade Proteases
Protease and Phosphatase Handbook

Detergent Handbook & Selection Guide

  • Proteomic Grade Detergents
  • Research Grade Detergents
  • Non-Ionic, Ionic & Zwitterionic
  • Detergent Estimations
  • Detergent Removal Systems
Detergent and Detergent Removal Handbook

Protein Electrophoresis Handbook

  • Gel Preparation Chemicals
  • Protein Marker Ladders
  • Electrophoresis Buffers
  • Reducing & Alkylating Reagents
  • Protein Gel Stains
Protein Electrophoresis Handbook and Selection Guide

Western Blotting & Electrophoresis Handbook

  • <90 Minute Western System
  • Transfer Buffers & Membranes
  • Membrane Stains
  • Blocking Buffers
  • Secondary Antibodies
  • Detection Reagents
  • Reprobing Reagents
Western Blotting & Electrophoresis Handbook

Mass Spectrometry Sample Preparation Handbook

  • Protein Sample Preparation
  • Protein Clean-Up Systems
  • Electrophoresis Reagents
  • Mass Spec Grade Protease
  • InGel Digestion Kits
  • Peptide Generation Reagents
Mass Spectrometry Sample Preparation Handbook

Protein Purification Handbook

  • Affinity Resins
  • 6X His Protein Purification Kits
  • GST Protein Purification Kits
  • Antibody Purification
  • Activated Resins
  • Buffers & Reagents
Protein Purification Handbook

Protein Labeling & Conjugation Handbook

  • Biotin Labeling
  • Cell Surface Protein Labeling
  • Agarose Coupling Kits
  • Fluorescent Dye Labeling Kits
  • Enzyme Labeling Systems
Protein Labeling & Conjugation Handbook

Antibody Production Handbook

  • Carrier Proteins
  • Peptide Coupling Systems
  • Antibody Purification Resins
  • Antibody Fragmentation Kits
Antibody Production Handbook

Assay Development Handbook

  • Coated Plates
  • Blocking Buffers
  • Wash Buffers
  • Secondary Antibodies
  • Detection Reagents
  • Antibody Labeling Systems
Assay Development Handbook

Protein Cross-Linkers Handbook

  • Homobifunctional 
  • Heterobifunctional
  • Optimizer Systems
  • Cross-Linking Systems
Protein Cross-Linking Handbook

BioAssays Handbook

  • Apoptosis Assays
  • Cytotoxicity Assays
  • SAM Methyltransferase Assays
  • Protease Assays
  • Phosphatase Assays
  • Peroxide Assay
  • ELISA
Bioassays Handbook

Molecular Biology Handbook

  • DNA Isolation
  • Transformation & Screening
  • Polymerase Chain Reaction
  • Agarose Electrophoresis
  • RNA Isolation
  • Yeast Transformation
Molecular Biology Handbook

Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers

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Ellyn Daugherty's Biotechnology: Science for the New Millennium

CB™ PROTEIN ASSAY: A Bradford Protein Assay

CB Protein Assay Graph

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay. This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein.

Features

  • Sensitivity: Linear responses over the range of 0.5µg-50µg protein
  • Flexible Protocols: Suitable for tube or Titer plate assays
  • Ready to use assay reagents and no preparation required
  • Long shelf life, stable for 12 months
Click for CB Protein Assay