The Protein Man's Blog | A Discussion of Protein Research

Ammonium Sulfate Protein Precipitation: The key to Salting-Out

Posted by The Protein Man on Sep 6, 2017 2:30:00 PM

Salt precipitation can be a very powerful tool to purify proteins by precipitation. Ammonium sulfate is usually the salt of choice since it is cheap, very soluble in water, and is able to become much more hydrated (interacts with more water molecules) than almost any other ionic solvent. In practice, ammonium sulfate is either added directly as a solid or added as a (usually) saturated solution to precipitate desired proteins.

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Topics: Protein Purification, Protein Concentration

Protect proteins with cryoprotectants & protein concentration

Posted by The Protein Man on Aug 1, 2017 2:30:00 PM

Rough handling and proteins do not necessarily go well together. As proteins are extremely sensitive and can easily be degraded during the process of concentration, purification, and storage, they need to be handled with utmost care to reduce, if not eliminate, the risk of unwanted aggregation and/or degradation.

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Topics: Protein Concentration

Key Protein Concentration & Enrichment Techniques | G-Biosciences

Posted by The Protein Man on Apr 18, 2017 2:30:00 PM

What is protein enrichment and why is there a need for it? Basically, protein enrichment is a technique where proteins of interest in a biological sample are concentrated to make them more suitable for identification and downstream analysis.

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Topics: Protein Concentration, Protein Fractionation, Protein Extraction

Magnetic Beads for Immunoprecipitation

Posted by The Protein Man on Nov 18, 2014 9:00:00 AM

Researchers have traditionally used agarose beads for immunoprecipitation (IP). However, there has been a growing trend in recent years favoring the use of magnetic beads. According to a recent survey of 1,013 scientists, 60% of the respondents will start using magnetic technology in the next three years.

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Topics: Protein Concentration, Antibody Production, Protein Extraction

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