The Protein Man's Blog | A Discussion of Protein Research

ELISA Principles: Explained

Posted by The Protein Man on Sep 13, 2022 11:15:00 AM

What is ELISA?

ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique designed to detect and quantify target molecules such as peptides, proteins, antibodies, and hormones in biological samples. Like other types of immunoassays, ELISA relies on specific antigen-antibody interactions to detect a target antigen.

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Topics: Assay Development (ELISA)

Mitochondria, Reactive Oxygen Species (ROS), and Their Effect on the Body

Posted by The Protein Man on Feb 23, 2021 4:30:00 PM

Reactive oxygen species (ROS) are highly reactive molecules and free radicals formed as a natural byproduct of oxygen metabolism. These molecules, which are present at low levels in normal cells, play a vital role in maintaining healthy redox processes and ensuring proper function in the cells. However, an overproduction of ROS may cause oxidative stress, cellular damage, and DNA damage, which may lead to several physiological and pathological conditions.

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Topics: Molecular Biology, Protein Electrophoresis, Protein Estimation, Assay Development (ELISA), Protein Concentration, Bioassays

The Amazing Origins of Antibody Diversity

Posted by The Protein Man on Sep 15, 2020 3:00:00 PM

The Origins of Antibody Diversity

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Topics: Molecular Biology, Western Blotting, Assay Development (ELISA)

Exploring the Genetic Causes of ALS (Lou Gehrig's Disease)

Posted by The Protein Man on Aug 4, 2020 3:30:00 PM

Despite the advances in modern medicine, no one knows for sure what causes amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease. Scientists can only theorize and speculate as to what causes it, but they still don’t have a conclusive answer to this question.

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Topics: Protein Purification, Western Blotting, Assay Development (ELISA), Sample Clean Up, Protein Concentration, Protein Extraction, Protein Detection

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