The Protein Man's Blog | A Discussion of Protein Research

Bradford Assay for Protein Concentration

Posted by The Protein Man on Dec 3, 2019 2:30:00 PM

The Bradford assay is a fast and efficient colorimetric assay to determine the protein concentration in a wide range of solutions and is widely used for protein content of cell fractions and protein concentrations for gel electrophoresis. It is often done before an SDS-PAGE analysis. Using the reagent Coomassie Brilliant Blue G-250, the solution undergoes a visible change in color when the dye binds to proteins via van de Waals and electrostatic interactions, causing a shift from a dark red to a deep blue. This is a result of stabilization moving the absorbance maximum of the dye from 470 to 595 nm. Tested samples typically take less than five minutes to reach the indicative measures of 595 nm absorbance concentration, making the Bradford assay both reliable and quick.

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Topics: Protein Estimation

The Difference Between the BCA and Bradford Protein Assays

Posted by The Protein Man on Oct 15, 2019 2:30:00 PM

When conducting an experiment, it is essential to choose the correct protein assay.

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Topics: Protein Estimation

Why Is Bovine Serum the Preferred Standard for Protein Assays?

Posted by The Protein Man on Sep 10, 2019 4:36:00 PM

Protein assays are one of the most widely used scientific methods, particularly in life sciences. An assay is an efficient spectroscopic procedure that analyzes the concentration of protein in a solution, also referred to as protein concentration quantitation. Being able to measure this concentration is an integral part of any laboratory workflow involving protein extraction, purification, labeling, or analysis. It is necessary for an array of research, including processing protein samples for isolation, separation, and analysis by chromatographic, electrophoretic, and immunochemical techniques. There are different types of assays and each can be useful for different applications, but many assays work in similar ways and utilize standards.

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Topics: Protein Estimation

How to Select the Best Protein Assay

Posted by The Protein Man on Aug 27, 2019 2:30:00 PM

With so many protein assays to choose from, each with its own advantages and limitations, you have to consider their suitability for the application. In some instances, it can be necessary to utilize more than one type of assay. Here, we discuss how to select the most appropriate protein assay.

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Topics: Protein Estimation

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