The Protein Man's Blog | A Discussion of Protein Research

Exposure to Intestinal microbes in early life can influence development of thymic lymphocytes : A review of current literature

Posted by The Protein Man on Jun 23, 2020 10:11:22 AM

 

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Topics: Western Blotting, Protein Electrophoresis, Protein Estimation, Protein Concentration, Protease Inhibitors, Protein Fractionation, Protein Labeling

How to Calculate Protein Concentration Using The Bradford Assay

Posted by The Protein Man on May 20, 2020 10:59:12 AM

Question:

How to Calculate Protein Concentration Using The Bradford Assay?  

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Topics: Protein Estimation

Bradford Assay for Protein Concentration | G-Biosciences

Posted by The Protein Man on Dec 3, 2019 2:30:00 PM

The Bradford assay is a fast and efficient colorimetric assay to determine the protein concentration in a wide range of solutions and is widely used for protein content of cell fractions and protein concentrations for gel electrophoresis. It is often done before an SDS-PAGE analysis. Using the reagent Coomassie Brilliant Blue G-250, the solution undergoes a visible change in color when the dye binds to proteins via van de Waals and electrostatic interactions, causing a shift from a dark red to a deep blue. This is a result of stabilization moving the absorbance maximum of the dye from 470 to 595 nm. Tested samples typically take less than five minutes to reach the indicative measures of 595 nm absorbance concentration, making the Bradford assay both reliable and quick.

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Topics: Protein Estimation

The Difference Between the BCA and Bradford Protein Assays

Posted by The Protein Man on Oct 15, 2019 2:30:00 PM

When conducting an experiment, it is essential to choose the correct protein assay.

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Topics: Protein Estimation

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