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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers
Poor Quality Detergents Cause Artifactual Results
How Do Detergents Work?
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Topics: Detergents, Protein Extraction
Question
I am looking to isolate proteins stuck to membranes in human and animal brain. I am then running the protein I retrieve on 2D gels. At the moment I am using a crude method to retrieve these proteins. This method is very time consuming and lacks reproducibility.
Do you have advice for isolating membrane proteins as well as aggregated protein stuck to the membrane for their subsequent analysis by 2D protein electrophoresis? Can I resuspend the proteins straight into my buffer for rehydration and IEF?
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Topics: Protein Extraction
