The Protein Man's Blog | A Discussion of Protein Research

Molecular Beacons: Dual Labeled Probes

Posted by The Protein Man on Apr 9, 2019 2:30:00 PM

Transcript analysis and DNA information is a crucial step in the field of molecular biology and genetics. For many years, DNA hybridization is utilized as a primal technique for diagnosis of genetic manipulations and their correlation with the diagnostic manifestations of the genetic diseases. The identification of DNA or RNA has become pivotal in many clinical studies and drug development approaches.

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Topics: Protein Detection

Antibody Selection for Immunohistochemistry

Posted by The Protein Man on Mar 26, 2019 1:18:00 PM

Antibodies have become a great tool for identifying and characterization of different proteins in different model systems using the technique of immunohistochemistry and Western blotting. The precision and sensitivity of these molecules allow us to develop a clear-cut picture of molecular cascades and signalling pathways. The technique of characterization of proteins by immunohistochemistry is very old, however it requires elaborate standardization and rigorous scientific approach to select the right antibodies for the experimental need.  

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Topics: Protein Labeling, Protein Detection

A Guide to Sample Preparation for Immunofluorescence

Posted by The Protein Man on Mar 12, 2019 2:30:00 PM

Immunofluorescence is an essential and a powerful tool in molecular biology for the visualization of cellular structures, protein localization and intracellular processes. The resultant sample produced after immunofluorescence is analyzed using different microscopic methods such as confocal microscopy, epifluorescence, TIRF, or GSDIM depending on the experiment.

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Topics: Protein Detection

Host Cell Protein Analysis: Detection in Biotherapeutics

Posted by The Protein Man on Feb 12, 2019 2:30:00 PM

Host cell proteins (HCPs) are one of the main impurities in bio-therapeutics that are carried over from the host expression system. Although HCPs are at low levels, they can trigger immunogenic reactions in the human system when administered along with bio-therapeutics. The similarity of physicochemical properties of HCPs and therapeutic proteins result in co-purification. Detection and elimination of these HCPs during bio-therapeutics purification are required. Development of analytical tools and methods for the detection of these HCPs during protein purification and quality control is a challenge. The final threshold level of HCPs varies for different bio-therapeutics, so manufacturers have
to analyze and report the HCP levels at all stages of downstream processing before the
product is released according to ICH-Q6B guidelines.

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Topics: Protein Detection

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