The Protein Man's Blog | A Discussion of Protein Research

What is the Link between Protein Folding and Prion Disease?

Posted by The Protein Man on Aug 12, 2020 5:00:00 PM



Prion diseases (also referred to as transmissible spongiform encephalopathies, or TSEs) are a group of progressive, untreatable, and fatal neurodegenerative conditions affecting both humans and animals. Generally, these conditions are characterized by long incubation periods (usually between 5 to 20 years), disruption in the normal tissue structure (resulting in holes and vacuole formation in the neurons), and the absence of an inflammatory response.

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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Cytotoxicity Assays, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Buffers & Chemicals

The Use of Spectral Scanning in Nucleic Acid Purity Assessment

Posted by The Protein Man on Jun 9, 2020 12:28:00 PM

 

Spectrophotometric analysis, also called spectral scanning, of biomolecules serves two main purposes – the quantitation of nucleic acids (DNA and RNA) and purity assessment. Since the amount or concentration and purity of the DNA or RNA in a solution significantly affect the results of subsequent downstream applications, establishing these values early on can reduce, if not totally eliminate, the risk of committing errors and guarantee optimum results.

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Topics: Molecular Biology, Apoptosis Assays, Assay Development (ELISA), Detergents, Buffers & Chemicals, Cytology & Histology

Biological Buffers: pH Range and How to Prepare Them

Posted by The Protein Man on May 13, 2014 6:54:00 AM

Question:

How to prepare biological buffers?  

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Topics: Buffers & Chemicals

Preparing Phosphate Buffers: How to Do It Right

Posted by The Protein Man on Sep 24, 2013 5:00:00 AM

Question:

How do you prepare your phosphate buffer?

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Topics: Buffers & Chemicals

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