The Protein Man's Blog | A Discussion of Protein Research

Lets Talk About Inclusion Bodies

Posted by The Protein Man on Mar 23, 2021 2:00:00 PM


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Topics: Protein Purification, Western Blotting, Protein Electrophoresis, Protein Estimation, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Extraction, Buffers & Chemicals, Protein Detection

Guess what??? Immobilized Papain Generates Fab Fragments of Cluster A Monoclonal Antibodies for Validation of Inner Domain of HIV-1 gp120?

Posted by The Protein Man on Aug 19, 2020 3:15:00 PM


The lab of Dr. William D. Tolbert and Dr. Neelakshi Gohain use Papain-linked agarose resin to create combinations of purified Fabs of Cluster A monoclonal antibodies (A32 mAbs and JR4 mAbs) to validate the design and crystal structure of a stabilized inner domain (ID) of HIV-1 gp120. This ID displays a major antibody-dependent cellular cytotoxicity (ADCC) target of the A32 region. Current research supports a role for ADCC in preventing HIV-1 infection and in vaccine-induced protection that correlates with slower HIV progression or decreased virus replication.

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Topics: Molecular Biology, Protein Estimation, Protein Concentration, Protein Labeling, Buffers & Chemicals

What is the Link between Protein Folding and Prion Disease?

Posted by The Protein Man on Aug 12, 2020 5:00:00 PM

Prion diseases (also referred to as transmissible spongiform encephalopathies, or TSEs) are a group of progressive, untreatable, and fatal neurodegenerative conditions affecting both humans and animals. Generally, these conditions are characterized by long incubation periods (usually between 5 to 20 years), disruption in the normal tissue structure (resulting in holes and vacuole formation in the neurons), and the absence of an inflammatory response.

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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Cytotoxicity Assays, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Buffers & Chemicals

The Use of Spectral Scanning in Nucleic Acid Purity Assessment

Posted by The Protein Man on Jun 9, 2020 12:28:00 PM


Spectrophotometric analysis, also called spectral scanning, of biomolecules serves two main purposes – the quantitation of nucleic acids (DNA and RNA) and purity assessment. Since the amount or concentration and purity of the DNA or RNA in a solution significantly affect the results of subsequent downstream applications, establishing these values early on can reduce, if not totally eliminate, the risk of committing errors and guarantee optimum results.

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Topics: Molecular Biology, Apoptosis Assays, Assay Development (ELISA), Detergents, Buffers & Chemicals, Cytology & Histology

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