The Protein Man's Blog | A Discussion of Protein Research

What is the Best Blocking Agent for Western Transfer?

Posted by The Protein Man on Sep 28, 2012 10:05:00 AM

Question:

Which Blocking Agent should I use to block non-specific binding during Western blotting?  

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Topics: Western Blotting

How is Protein Electrophoresis Different from Western Blotting?

Posted by The Protein Man on Aug 6, 2012 5:00:00 AM

Question:

What is the difference between Protein Electrophoresis and Western Blotting?  

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Topics: Western Blotting, Protein Electrophoresis

14 Must Have Life Science Handbooks

Posted by The Protein Man on May 4, 2012 1:18:00 PM

Click the covers to download the Handbooks

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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers

7 Common Problems with Western transfer Solved

Posted by The Protein Man on Mar 21, 2012 10:06:00 AM

The aim of this blog is to highlight some of the routine problems that occur with Western blotting and suggest possible solutions.  The 7 common problems are:

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Topics: Western Blotting, Protein Electrophoresis

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