Question:
What is the role of the isoelectric point of a protein in its purification and how does it come into play with your protein purification and extraction buffer products?
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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers
Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin. Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH.
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Topics: Protein Purification, Protein Labeling
