Question:
What is the role of protein fractionation in protein research?
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Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Protein Estimation, Apoptosis Assays, Cytotoxicity Assays, Assay Development (ELISA), Detergents, Sample Clean Up, Protease Inhibitors, Antibody Production, Protein Fractionation, Protein Labeling, Protein Extraction, Mass Spectrometry, Cross-Linkers
Pitfalls of Protein Identification
Proteomics is the analysis of a species' protein complement that is expressed by its genome, but the sheer complexity of the proteome raises significant issues for a researcher. There are an estimated 20,000-25,000 human protein-coding genes that could code for >1,000,000 proteins, especially when protein modifications are considered. These include N- and C-truncations, alternative spliced forms and the large number of Co- and Post-translational modifications, including:
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Topics: Protein Fractionation
