The Protein Man's Blog | A Discussion of Protein Research

What is the Link between Protein Folding and Prion Disease?

Posted by The Protein Man on Aug 12, 2020 5:00:00 PM



Prion diseases (also referred to as transmissible spongiform encephalopathies, or TSEs) are a group of progressive, untreatable, and fatal neurodegenerative conditions affecting both humans and animals. Generally, these conditions are characterized by long incubation periods (usually between 5 to 20 years), disruption in the normal tissue structure (resulting in holes and vacuole formation in the neurons), and the absence of an inflammatory response.

Read More

Topics: Protein Purification, Molecular Biology, Western Blotting, Protein Electrophoresis, Cytotoxicity Assays, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Buffers & Chemicals

Cells of the Immune System

Posted by The Protein Man on Jul 22, 2020 2:57:00 PM

Antibodies3-resized-600

 

Read More

Topics: Protein Purification, Molecular Biology, Assay Development (ELISA), Sample Clean Up

How the cilium became a ‘villain’ in Apicomplexan parasites?

Posted by The Protein Man on Jul 15, 2020 1:45:00 PM

Evolution of ancient cellular components paves a path to parasitic adaptations in Apicomplexan parasites.

Read More

Topics: Molecular Biology, Protein Electrophoresis, Protein Concentration, Protease Inhibitors, Protein Labeling

The Use of Spectral Scanning in Nucleic Acid Purity Assessment

Posted by The Protein Man on Jun 9, 2020 12:28:00 PM

 

Spectrophotometric analysis, also called spectral scanning, of biomolecules serves two main purposes – the quantitation of nucleic acids (DNA and RNA) and purity assessment. Since the amount or concentration and purity of the DNA or RNA in a solution significantly affect the results of subsequent downstream applications, establishing these values early on can reduce, if not totally eliminate, the risk of committing errors and guarantee optimum results.

Read More

Topics: Molecular Biology, Apoptosis Assays, Assay Development (ELISA), Detergents, Buffers & Chemicals, Cytology & Histology

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium