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The Protein Man's Blog | A Discussion of Protein Research

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Protein Analysis and Identification by Mass Spectrometry: Taking a Closer Look

  
  
  
mass spectrometry, protein analysis

Question:

Can mass spectrometry help in protein analysis and identification?  

Protein Extraction and Isolation Accessories for Sample Preparation

  
  
  
sample preparation, protein extraction

Question:

What protein extraction and isolation accessories are used for sample preparation?

Uncovering the Importance of Detergents in the Study of Membrane Proteins

  
  
  
detergents, membrane protein

Question:

What is the role of detergents in the study of membrane proteins?  

Tags: 

Solubilized Proteins: Understanding Their Importance

  
  
  
solubilized protein, protein solubilization

Question:

What are some important applications of solubilized proteins?  

Sample Preparation for Western Blotting: A Definitive Guide

  
  
  
western blotting, sample preparation

Question:

How to prepare sample for western blotting?  

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

  
  
  
Vmax Determination

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

  
  
  
protein detection, western blotting

Question:

How to detect protein using western blotting?  

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)

  
  
  
protein detection

Question:

How to detect protein using western blotting?  

What Assay Development Accessories Should You Use For Protein Binding? (Part 3 f 3)

  
  
  
assay development, protein estimation

Question:

What assay development accessories should be used for protein estimation?  

What Assay Development Accessories Should You Use For Protein Binding? (Part 2 f 3)

  
  
  
assay development, protein estimation

Question:

What assay development accessories should be used for protein binding?  

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