The Protein Man's Blog | A Discussion of Protein Research

Western Blot Blocking: Tips and Tricks

Posted by Protein Man on Feb 22, 2017 2:25:00 PM


Western blotting is an immunodetection technique used to first separate proteins by gel electrophoresis then to transfer those separated proteins from the gel matrix onto a secondary, more durable matrix, most commonly polyvinylidene difluoride (PVDF) or nitrocellulose.

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Topics: Western Blotting

Cell Lysis: 5 Common Cell Disruption Methods | G-Biosciences

Posted by Protein Man on Feb 14, 2017 2:33:00 PM

When working on proteins, your sample biological materials should first be homogenized to ensure proper solubilization and extraction. This can be a challenge if you are trying to extract plant proteins because their tissues contain significant amounts of proteases, phosphatases and glycosidases which can interfere with the results. In addition, not all biological materials require the same technique so you should really be careful when choosing the technique that you would use.

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Topics: Protein Extraction

Detergents: Ionic, Non-Ionic, and Zwitterionic. What's the Difference?

Posted by Protein Man on Feb 8, 2017 2:05:00 PM

There is a plethora of uses for detergents, both in the lab and in everyday life. The usefulness of these cleaning compounds comes from subtle differences in their chemical structure. Detergents are composed of amphipathic molecules, containing a polar hydrophilic head group attached to a long-chain hydrophobic carbon tail. The composition and charge of the molecules’ head and tail groups determines the mechanism by which these molecules will act as detergents. Because of this, different types of detergents are useful for a variety of purposes. 

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Topics: Detergents

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