New Call-to-action

Subscribe via E-mail

Technical Handbooks

HB-Protein-Assays-HP

The Protein Man's Blog | A Discussion of Protein Research

Why BCA Protein Assay is Overhyped

Posted by Protein Man on Jul 3, 2015 10:00:00 AM

The bicinchroninic acid (BCA) assay, also known as the Smith assay, is a biochemical assay used to determine the total concentration of protein in a solution. Due to its ability to provide accurate determination of protein concentration and its compatibility with most protein sample types, protein laboratories around the world prefer the BCA assay over any other detergent-compatible assays.

Read More

Are Total Protein Membrane Stains Compatible With IR Imaging Systems?

Posted by Protein Man on Jun 29, 2015 2:36:00 PM

We recently reviewed the advantages of total protein membrane stains as a loading control for Western blotting and demonstrated how they were an improvement on routinely used housekeeping genes.

What's best? Housekeeping genes or total protein for Western blot loading controls

 

Read More

Tags: Western Blotting

What's best? Housekeeping genes or total protein for Western blot loading controls

Posted by Protein Man on Jun 29, 2015 2:34:00 PM

Western blotting is a very popular and sensitive protein detection system that is routinely used for multiple areas of protein research.  The sensitivity of this detection system continues to improve with increasingly sensitive enhanced chemiluminescence systems and the increasing popularity of IR (infra-red) fluorophores and IR imaging systems:

Read More

Tags: Western Blotting, Protein Detection

Enhanced Chemiluminescence: What is it?

Posted by Protein Man on Jun 29, 2015 10:00:00 AM

Due to its high sensitivity, wide dynamic range, and high signal-to-noise ratio, enhanced chemiluminescence or ECL is considered as one of the most popular detection methods for a variety of western blotting applications in most protein laboratories around the world. This method also proves to be very useful in the quantification of a wide variety of biological materials such as cells, proteins, RNA, DNA, and a host of other analytes.

Read More

Tags: Western Blotting

How to Deal with Interfering Agents in Protein Estimation Assays

Posted by Protein Man on Jun 10, 2015 10:00:00 AM

Protein quantitation or estimation assays are widely used for determining protein concentration and are considered to be one of the most widely used methods in life science research. Estimation of protein concentration is necessary in protein purification, electrophoresis, cell biology, molecular biology, and other research applications.

Read More

Coomassie, Enhanced Coomassie, and Colloidal Coomassie: What’s the Difference?

Posted by Protein Man on Jun 4, 2015 10:00:00 AM

Among all the protein staining techniques available today, most researchers around the world prefer using Coomassie dyes (also known as Coomassie brilliant blue) in visualizing electrophoresed proteins. There are a number of good reasons why they do. Here are some of them:

Read More

Tags: Protein Detection

How to Choose between G250 or R250 Coomassie Dyes

Posted by Protein Man on Apr 22, 2015 10:00:00 AM

11586304016_90f3ded54b_z_1Proteins separated by gel electrophoresis can be visualized using different staining procedures, including Coomassie stains, silver stains and fluorescent stains. Among these staining techniques, silver staining undoubtedly offers the highest sensitivity. However, it also involves a complex and relatively time-consuming protocol, has a low linear dynamic range and reproducibility, and is not compatible with mass spectrometry.

Read More

Dialysis - Knot the Way to Do It

Posted by Protein Man on Apr 17, 2015 10:00:00 AM

Dialysis_-_Knot_the_Way_to_Do_ItThere are certain things that you should not do when performing dialysis in the laboratory. To ensure that all unwanted small molecular weight substances (such as reducing agents, non-reacted crosslinking or labeling reagents, and preservatives) are removed and facilitate a more efficient buffer exchange for macromolecular samples such as proteins, here are some of the things you need to avoid at all cost.   

Read More

Tags: Sample Clean Up

Why SDS is a Necessary Evil in PAGE

Posted by Protein Man on Apr 2, 2015 9:00:00 AM

6876898938_46a67873fc_z1lSodium dodecyl sulphate-polyacrylamide gel electrophoresis or SDS-PAGE is commonly used in protein aboratories for separating proteins based on their molecular weights. This is accomplished by taking advantage of their differential rates of migration through a sieving matrix (the acrylamide or agarose gel) in response to an applied electrical field.

Read More

Colorimetric or Copper Based Protein Assays - How to Decide Which is Best

Posted by Protein Man on Mar 26, 2015 10:00:00 AM

While protein assays have a variety of uses in life science research, there is no single assay method that is suitable for all applications.  Despite all the advancements in modern science, a protein assay method that is not affected by any non-protein components or by the differences in protein composition does not exist. For this reason, protein laboratories find it necessary to have more than one type of protein assay for research applications.

Read More

Tags: Protein Estimation

Subscribe via E-mail

Browse by Tag

Follow The Protein Man!

Technical Handbooks

HB-Protein-Assays-HP