ask-protein-man-banner

Subscribe via E-mail

Your email:

Follow The Protein Man!

Technical Handbooks

hb-protein-assays-hp

The Protein Man's Blog | A Discussion of Protein Research

Current Articles | RSS Feed RSS Feed

How Proteins Interact with DNA and RNA to Influence Nucleic Acid

  
  
  
New Picture (11)

Due to the fact that nucleic acids carry genetic information and that proteins regulate various life processes, they are considered to be two of the most important biomolecules in any living organism. In addition, their interactions play a crucial role in most biological processes, which include everything from replication,transcription and recombination to enzymatic eventsusing nucleic acids as substrates. Taking all of these things into consideration, it is not surprising why protein-nucleic acids interactions have been the subject of intensive research for the past few years.

What You Need to Know About NTA and IDA Ligands

  
  
  
New Picture (10)

Since 1975, immobilized metal affinity chromatography (IMAC) has been popularly used in purifying proteins, especially those that are fused to a polyhistidine tag, typically a 6X His tag. This process gained immense popularity since it allows for the efficient purification of proteins, even those from crude lysates. In addition, its robust nature makes it ideal for methods that require protein-specific conditions. Its functional simplicity, affordability and compatibility with a wide range of reagents also add to its popularity.

A Guide to Protein Cross Linkers

  
  
  
protien11

Protein crosslinking reagents or crosslinkers can be accurately defined as molecules containing two or more reactive ends that are capable of chemically attaching to specific functional groups on proteins or other molecules. These reagents are generally used in creating detectable scientific probes to facilitate a number of proteomics methods, including Western blotting, ELISA and other strategies for studying protein-protein interactions.

Interfering Agents of 2D Electrophoresis and How to Remove Them

  
  
  
describe the image

Most biological samples contain non-protein substances and/or contaminants that may interfere with the resolution of the electrophoretic separation. Considering the fact that cell lysates usually contain macromolecules and small ionic molecules which may interfere with the electrophoretic process, these agents should be eliminated prior to electrophoresis, especially if their amount exceeds the critical interference threshold.

Special Considerations When Extracting Plant Proteins

  
  
  
Protein extractionThere is simply no other way around it. You need to break down the walls (the cell wall, that is) to extract the good stuff. While you can easily accomplish this task when extracting proteins from mammalian cells (they have no cell walls to begin with), it can be more difficult when you are working with plants, yeast, bacteria, fungi and Archaea. These organisms have rigid cell walls that protect the basic cell structure against destructive mechanical forces.

8 Protein Tags Explained

  
  
  
Protein TagsBasically, protein tags are peptide sequences that are attached to proteins to facilitate easy detection and purification of expressed proteins. In addition, they can also be used to identify potential binding partners for your protein of interest.

Dialysis in Protein Research: Understanding the Basics

  
  
  
dialysis, protein research

Question:

How dialysis is used in protein research?  

How to clean the sinter of a Büchner flask?

  
  
  

Are the sinters of frits of your funnels and glassware blocked? Is the flow-through extremely slow or retarded?

Biological Buffers: pH Range and How to Prepare Them

  
  
  
biological buffers resized 600

Question:

How to prepare biological buffers?  

Determining Protein Molecular Weight with SDS-PAGE: An Overview of the Process

  
  
  
Molecular Weight Determination

Question:

How to Determine Molecular Weight of Protein with SDS-Page?

All Posts