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Protein Lysis Buffer 101 –Components and Functions (Part 1)

  
  
  
protein lysis buffer

Question:

What are the components of a protein lysis buffer and what are their primary functions?  

How to Reduce Pipetting Errors When Working with Apoptosis Assays

  
  
  
Working with Apoptosis Assays

Question:

Improve your pipetting techniques to get accurate results when working with apoptosis assays

DNA Purification: Which Solvent Should You Use?

  
  
  
dna purification

Question:

Which is Better Choice for DNA Purification in Ethanol vs. Isopropanol?  

Using Ampicillin in Plasmid DNA Isolation: What You Need to Know

  
  
  
describe the image

Question:

What you need to know about Ampicillin and Plasmid DNA Isolation?  

Protein Immunoprecipitations: Which method to use?

  
  
  
Classic Immunoprecipitation

Traditional Immunoprecipitation procedure can be divided into four key steps (Figure 1):

DNA Purification vs. RNA Purification: What is the Difference?

  
  
  
dna purification, rna purification

Question:

What is the Difference between DNA Purification and RNA Purification?

A More In-Depth Look at the Classification of Cytotoxicity Assays

  
  
  
cytotoxicity assays

Question:

Cytotoxicity Assays: How Are They Classified?  

Assay Development 101: A Beginner’s Guide

  
  
  
assay development

Question:

What you need to know about assay development?  

Understanding the Role of Proteases in DNA Extraction

  
  
  
dna extraction, molecular biology

Question:

What is the role of proteases in DNA extraction?  

Sepharose or Agarose for protein resins, which should I choose?

  
  
  
Sepharose or agarose

The answer is simple. It really doesn't matter as they are in fact the same.

Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed.  Its brand name is derived from Separation-Pharmacia-Agarose.

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