In gene expression research, analysis starts with one critical step: transforming RNA into complementary DNA (cDNA). The quality of the resulting cDNA impacts the accuracy and reliability of every downstream technique, from amplification to quantification. Even the most advanced analytical methods will fall short if the quality of starting material is lacking.
Topics: complementary DNA (cDNA), Reverse Transcriptase (RT), Oligo (dT) primer, RT-qPCR, Fidelity and Processivity of enzymes, Random hexamer, Thermostable enzymes, RNA
Gene expression is the fundamental process by which the genetic code within DNA is translated into functional proteins, the workhorses of the cell. This intricate process involves two key steps: transcription (where DNA is transcribed into RNA) and translation (where RNA is translated into protein). Understanding the journey from RNA to protein is not only a cornerstone of molecular biology but also a critical area of research for advancements in medicine, biotechnology, and synthetic biology.
Topics: Translation, Taq DNA Polymerase, quantitative Polymerase Chain Reaction (qPCR), Gene expression, Transcription, complementary DNA (cDNA), Reverse Transcriptase (RT)
