The Protein Man's Blog | A Discussion of Protein Research

This guide applies to many, but not all antibodies.  Always consult the product insert of the antibody for storage and handling instructions.

Blocking buffers are used to bind the surface areas that may attach to reagents during the testing process and prevent nonspecific antibodies from interacting with reagents. Blockers can also cause poor reading or inaccurate results.

As a common technique in molecular and cell biology, Western blot analysis allows you to detect specific proteins in a tissue homogenate or extract. However, if you want to ensure the success of your Western blot experiment, you need to learn how to properly prepare samples.