Subcellular Fractionation: ‘Exploring cells through centrifugation'
Topics: Protein Purification
Antibody Purification by Affinity Chromatography
The availability of purified antibodies is essential to various applications. While there are various methods of purification, affinity chromatography is the most popular antibody purification technique.
Topics: Protein Purification, Antibody Production
In 1975, Porath developed Immobilized Metal Affinity Chromatography (IMAC). It is based on the interaction of specific protein residues, including histidines, cysteines, and occasionally, tryptophans, with cations of transition metals.
Topics: Protein Purification
Capture and purification of low abundance proteins
A proteome is a sum total of all the cellular proteins, this would include housekeeping proteins and proteins that are produced in response to a stimulus. Some proteins are produced only in a given cell type or tissue, others are produced in all cells, some proteins are only produced under certain given conditions adding to the complexity and heterogeneity of proteins in a proteome. Similarly, alternative splicing and post translational modifications all together combined can make the proteome very complex.
Topics: Protein Purification, Protein Concentration, Peptide Generation