The Protein Man's Blog | Protein Purification (4)

Cells of the Immune System

Posted by The Protein Man on Jul 22, 2020 2:57:00 PM

Antibodies3-resized-600

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Topics: Protein Purification, Molecular Biology, Assay Development (ELISA), Sample Clean Up

Subcellular Fractionation: ‘Exploring cells through centrifugation'

Posted by The Protein Man on Jun 30, 2020 1:47:00 PM

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Topics: Protein Purification

Antibody Purification by Affinity Chromatography

Posted by The Protein Man on Apr 28, 2020 2:30:00 PM

The availability of purified antibodies is essential to various applications. While there are various methods of purification, affinity chromatography is the most popular antibody purification technique.

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Topics: Protein Purification, Antibody Production

Tips for Using Ni-NTA Resin

Posted by The Protein Man on Feb 25, 2020 2:30:00 PM

In 1975, Porath developed Immobilized Metal Affinity Chromatography (IMAC). It is based on the interaction of specific protein residues, including histidines, cysteines, and occasionally, tryptophans, with cations of transition metals.

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Topics: Protein Purification