The Protein Man's Blog | A Discussion of Protein Research

Cysteine is one of the most reactive amino acid residue owing to the thiol (-SH) group, thiols can serve as an electron donor in different reactions. Cysteine thiols can form part of catalytic centre and can frequently participate in enzymatic reactions, not just that, cysteine thiols can also undergo a variety of covalent post-translational modifications (PTMs) and formation of disulphide bonds. The thiol modifications can act as important mediators of redox signalling and protein function regulation.

Determining protein localisation and membrane topology

Flow cytometry (FCM) is a multi-parameter cell analysis technique used for detecting and measuring the physical and chemical properties of a particular cell population, including cell count, cell size, and cell cycle. While the method has been routinely used for basic cell biological research, it also plays a vital role in the diagnosis, treatment, and management of certain bone and soft tissue tumors and blood cancers such as leukemia, lymphoma, and myeloma.