The Protein Man's Blog | A Discussion of Protein Research

Cells of the Immune System

Posted by The Protein Man on Jul 22, 2020 2:57:00 PM

Antibodies3-resized-600

 

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Topics: Protein Purification, Molecular Biology, Assay Development (ELISA), Sample Clean Up

Antibody Basics Revisited (Do’s and Don’ts of Antibody Handling)

Posted by The Protein Man on Jun 16, 2020 1:00:00 PM

This guide applies to many, but not all antibodies.  Always consult the product insert of the antibody for storage and handling instructions.

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Topics: Western Blotting, Sample Clean Up, Antibody Production

How the ACE2 Receptor Enables SARS and COVID-19

Posted by The Protein Man on Apr 21, 2020 11:22:00 AM



With more than one million cases in 208 countries and territories around the world, the scientific and medical communities are on a race against time to find a viable prevention and treatment plan for the novel coronavirus (SARS-CoV-2) and its associated disease, COVID-19, which has already claimed tens of thousands of lives to date. This challenge has led them to focus on how the virus breaks into human cells.

How Does the Coronavirus Infect Target Cells?

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Topics: Molecular Biology, Sample Clean Up

Sputum Processing, from viscous mucus to homogenous samples

Posted by The Protein Man on Oct 10, 2017 2:32:00 PM

Sputum Liquefaction: from viscous, heterogeneous mucus to equivalent, homogenous samples

Sputum processing has one main purpose; to render the viscous, heterogeneous specimen to a homogenous state (evenly distributed suspension). This homogenous suspension will be dispensed into multiple aliquots for cryopreservation, smear microscopy, gram stain, and culture. Each aliquot should be representative of the original specimen. This can be accomplished through the process of sputum liquefaction.

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Topics: Sample Clean Up

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