The Protein Man's Blog | A Discussion of Protein Research

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

Posted by The Protein Man on Jan 29, 2014 1:56:00 PM

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.

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Topics: Apoptosis Assays, Assay Development (ELISA)

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Posted by The Protein Man on Jan 21, 2014 5:00:00 AM

Question:

How to detect protein using western blotting?  

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Topics: Western Blotting

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)

Posted by The Protein Man on Jan 14, 2014 5:00:00 AM

Question:

How to detect protein using western blotting?  

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Topics: Western Blotting

What Assay Development Accessories Should You Use For Protein Binding? (Part 3 f 3)

Posted by The Protein Man on Jan 7, 2014 5:00:00 AM

Question:

What assay development accessories should be used for protein estimation?  

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Topics: Assay Development (ELISA)

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