To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.
How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?
Posted by
The Protein Man on Jan 29, 2014 1:56:00 PM
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Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)
Posted by
The Protein Man on Jan 21, 2014 5:00:00 AM
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Topics: Western Blotting
Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)
Posted by
The Protein Man on Jan 14, 2014 5:00:00 AM
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Topics: Western Blotting
What Assay Development Accessories Should You Use For Protein Binding? (Part 3 f 3)
Posted by
The Protein Man on Jan 7, 2014 5:00:00 AM
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Topics: Assay Development (ELISA)