The Protein Man's Blog | A Discussion of Protein Research

Question:

What is the role of detergents in the study of membrane proteins?  

Question:

What are some important applications of solubilized proteins?  

Question:

How to prepare sample for western blotting?  

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.