The Protein Man's Blog | A Discussion of Protein Research

Which protein assay should you choose for your experiment – the BCA (Bicinchoninic Acid) protein assay or the Bradford protein assay? Since there is practically not a single protein assay method that is perfectly specific to particular proteins or sensitive to all protein types, your success will ultimately depend on three important factors:

As a functional unit of a living entity, cells are very complicated and consist of exceptionally high number of biomolecules with completely different physical and chemical properties. The complexity of the biomolecules profile is even more intricately woven depending upon their subcellular location. With the limited resolution of the separation and localization technologies, it is imperative to employ fractionation methods for protein profiling and expression analysis.  Even the lesser abundant proteins can be analysed if screened after prefractionation. Sensitive and accurately fractionated samples help in convolution of methods such as mass spectrometry or in-vitro analysis for the detection of proteins at the level of organelles or transportation compartments.

Transcript analysis and DNA information is a crucial step in the field of molecular biology and genetics. For many years, DNA hybridization is utilized as a primal technique for diagnosis of genetic manipulations and their correlation with the diagnostic manifestations of the genetic diseases. The identification of DNA or RNA has become pivotal in many clinical studies and drug development approaches.

Cell culture is extensively growing technique applied in various fields of basic research, regenerative medicines and biotechnological applications. The use of cells is exponentially increasing in wide-range of studies involving cell division, cell growth and proliferation, bioassay development and drug treatments. However, the credibility of results depends on the reproducibility of results and quality of cell culture.