The Protein Man's Blog | A Discussion of Protein Research

Many researchers use high pressure liquid chromatography (HPLC) to separate and measure concentrations of small molecules. However, this method is relatively ineffective when trying to extract proteins and other biochemicals. Organic solvents used in HPLC place restrictions on these biochemicals, which are unable to function at high temperatures. For this reason, in 1982, Pharmacia developed fast protein liquid chromatography (FPLC) to overcome the weaknesses of the HPLC and institute an effective, quick means to extract and purify biochemicals - especially proteins. 

 In a cell, protein degradation and synthesis maintain the balance of proteins, where old proteins are degraded into amino acids, which are further used in the synthesis of new proteins. Proteases/Peptidases play an important role in protein degradation. Proteases are present in almost all living cells. In a living cell, protease action is regulated by external as well as internal factors. Some proteases are inactive under normal conditions, but are activated only when necessary. For example, during apoptosis, Pro-caspase (inactive) converts to Caspase (active). Sub-cellular localization of proteases also helps in their regulated activity. Most of them are localized to sub-cellular organelles like lysosomes and thereby restricted their activity only to those proteins directed to lysosomes. 

Proteins undergo many kinds of post translational modifications (PTMs), such as phosphorylation, ubiquitination, SUMOylation, geranyl-geranylation, farnesylation, myristoylation, acetylation, succinylation and palmitoylation. PTMs can be reversible or irreversible, they can be dynamic or stable. PTMs enable fine tuning of protein function in response to various internal and external signals and therefore they have profound effects on cellular physiology, metabolism, survival and growth.

What is a Protease Inhibitor?

If you want to protect your target protein from potential harm, there is one thing you should do – use a protease inhibitor.