The Protein Man's Blog | A Discussion of Protein Research

There are various methods to enrich or purify proteins of interest from other proteins and components. Most purification methods involve a form of chromatography where molecules in a solution or mobile phase separate based on differences in chemical or physical interaction with a stationary material or solid phase.

As a common technique in molecular and cell biology, Western blot analysis allows you to detect specific proteins in a tissue homogenate or extract. However, if you want to ensure the success of your Western blot experiment, you need to learn how to properly prepare samples.

As a non-ionic viscous liquid, tweens are a class of emulsifiers used in pharmaceuticals and food preparation.

Analyzing the effects of necrosis and apoptosis is a vital component of biological research. Although there are numerous assays to detect apoptosis, there are significantly fewer assays available to measure necrosis. The release of LDH is a beneficial method for detecting necrosis, especially when combined with cellular cytotoxicity.