The Protein Man's Blog | A Discussion of Protein Research

Blocking buffers are used to bind the surface areas that may attach to reagents during the testing process and prevent nonspecific antibodies from interacting with reagents. Blockers can also cause poor reading or inaccurate results.

In 1975, Porath developed Immobilized Metal Affinity Chromatography (IMAC). It is based on the interaction of specific protein residues, including histidines, cysteines, and occasionally, tryptophans, with cations of transition metals.

The RIPA buffer, or Radioimmunoprecipitation assay buffer, is ideal for whole-cell extracts, as well as protein assays. However, it is an essential component in other applications too. 

The Roles of Trypsin in Protein Analysis

What is Trypsin?