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Solubilized Proteins: Understanding Their Importance

Posted by The Protein Man on Mar 5, 2014 5:00:00 AM
Solubilized Proteins: Understanding Their Importance

Question:

What are some important applications of solubilized proteins?  

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Topics: Sample Clean Up

Sample Preparation for Western Transfer: A Definitive Guide

Posted by The Protein Man on Feb 12, 2014 6:14:00 AM
Sample Preparation for Western Transfer: A Definitive Guide

Question:

How to prepare sample for western blotting?  

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Topics: Western Blotting

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

Posted by The Protein Man on Jan 29, 2014 1:56:00 PM
How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.

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Topics: Apoptosis Assays, Assay Development (ELISA)

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Posted by The Protein Man on Jan 21, 2014 5:00:00 AM
Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Question:

How to detect protein using western blotting?  

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Topics: Western Blotting

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