The Protein Man's Blog | A Discussion of Protein Research

The Protein Man

The Protein Man

Recent Posts

Sample Preparation for Western Transfer: A Definitive Guide

Posted by The Protein Man on Feb 12, 2014 6:14:00 AM
Sample Preparation for Western Transfer: A Definitive Guide

Question:

How to prepare sample for western blotting?  

Read More
8 Comments Click here to read/write comments

Topics: Western Blotting

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

Posted by The Protein Man on Jan 29, 2014 1:56:00 PM
How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.

Read More
0 Comments Click here to read/write comments

Topics: Apoptosis Assays, Assay Development (ELISA)

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Posted by The Protein Man on Jan 21, 2014 5:00:00 AM
Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 2)

Question:

How to detect protein using western blotting?  

Read More
0 Comments Click here to read/write comments

Topics: Western Blotting

Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)

Posted by The Protein Man on Jan 14, 2014 5:00:00 AM
Using Western Blotting for Protein Detection: Some Commonly Used Methods (Part 1)

Question:

How to detect protein using western blotting?  

Read More
1 Comment Click here to read/write comments

Topics: Western Blotting

All posts

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium

CONNECT WITH US

CONNECT

Stay up to date with G-Biosciences by signing up for our newsletter.

INFORMATION

CORPORATE

My Account

Pci

think proteins! think G-Biosciences! © 2019 Geno Technology Inc., USA. All Rights Reserved.