There is simply no other way around it. You need to break down the walls (the cell wall, that is) to extract the good stuff. While you can easily accomplish this task when extracting proteins from mammalian cells (they have no cell walls to begin with), it can be more difficult when you are working with plants, yeast, bacteria, fungi and Archaea. These organisms have rigid cell walls that protect the basic cell structure against destructive mechanical forces.
Special Considerations When Extracting Plant Proteins
Posted by
The Protein Man on Jun 17, 2014 10:01:00 AM
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Topics: Protein Extraction
Basically, protein tags are peptide sequences that are attached to proteins to facilitate easy detection and purification of expressed proteins. In addition, they can also be used to identify potential binding partners for your protein of interest.
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Topics: Protein Purification
Dialysis in Protein Research: Understanding the Basics
Posted by
The Protein Man on May 28, 2014 5:00:00 AM
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Topics: Protein Concentration
Are the sinters of frits of your funnels and glassware blocked? Is the flow-through extremely slow or retarded?
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