The Protein Man's Blog | A Discussion of Protein Research

What's best? Housekeeping genes or total protein for Western blot loading controls

Posted by The Protein Man on Jun 29, 2015 2:34:00 PM

Western blotting is a very popular and sensitive protein detection system that is routinely used for multiple areas of protein research.  The sensitivity of this detection system continues to improve with increasingly sensitive enhanced chemiluminescence systems and the increasing popularity of IR (infra-red) fluorophores and IR imaging systems:

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Topics: Western Blotting, Protein Detection

Enhanced Chemiluminescence: What is it?

Posted by The Protein Man on Jun 29, 2015 10:00:00 AM

Due to its high sensitivity, wide dynamic range, and high signal-to-noise ratio, enhanced chemiluminescence or ECL is considered as one of the most popular detection methods for a variety of western blotting applications in most protein laboratories around the world. This method also proves to be very useful in the quantification of a wide variety of biological materials such as cells, proteins, RNA, DNA, and a host of other analytes.

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Topics: Western Blotting

How to Deal with Interfering Agents in Protein Estimation Assays

Posted by The Protein Man on Jun 10, 2015 10:00:00 AM

Protein quantitation or estimation assays are widely used for determining protein concentration and are considered to be one of the most widely used methods in life science research. Estimation of protein concentration is necessary in protein purification, electrophoresis, cell biology, molecular biology, and other research applications.

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Topics: Protein Estimation

Coomassie, Enhanced Coomassie, and Colloidal Coomassie: What’s the Difference?

Posted by The Protein Man on Jun 4, 2015 10:00:00 AM

Among all the protein staining techniques available today, most researchers around the world prefer using Coomassie dyes (also known as Coomassie brilliant blue) in visualizing electrophoresed proteins. There are a number of good reasons why they do. Here are some of them:

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Topics: Protein Electrophoresis, Protein Detection

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