After the protein bands have been separated by electrophoresis, researchers perform a dye-binding or color-producing chemical reaction to visualize the proteins embedded within the pores of the polyacrylamide gel matrix. Some of the most popular in-gel detection methods include staining with Coomassie dye, metallic silver, and zinc.
Topics: Protein Electrophoresis, Protein Detection
The bicinchroninic acid (BCA) assay, also known as the Smith assay, is a biochemical assay used to determine the total concentration of protein in a solution. Due to its ability to provide accurate determination of protein concentration and its compatibility with most protein sample types, protein laboratories around the world prefer the BCA assay over any other detergent-compatible assays.
Topics: Protein Estimation
We recently reviewed the advantages of total protein membrane stains as a loading control for Western blotting and demonstrated how they were an improvement on routinely used housekeeping genes.
Topics: Western Blotting
Western blotting is a very popular and sensitive protein detection system that is routinely used for multiple areas of protein research. The sensitivity of this detection system continues to improve with increasingly sensitive enhanced chemiluminescence systems and the increasing popularity of IR (infra-red) fluorophores and IR imaging systems:
Topics: Western Blotting, Protein Detection
