The Protein Man's Blog | A Discussion of Protein Research

Gel Filtration and Its Role in Desalting and Buffer Exchange

Posted by The Protein Man on Jul 19, 2016 10:00:00 AM

Gel filtration is a method of separating molecules by size. Among similar techniques, gel filtration is known as being a very simple and gentle procedure. When it comes to desalting and buffer exchange, gel filtration is often the preferred method as it can achieve better separation without any complex processes. Gel filtration is commonly used for chromatography, with the only negative being that it is very difficult to get high resolution results.

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Fast Flow Agarose Resins: What's The Real Difference?

Posted by The Protein Man on Jul 14, 2016 10:00:00 AM

For the purposes of chromatography, fast flow agarose resin provides a stable, easy, and effective medium. Comprised of cross-linked agarose beads, fast flow agarose resin is able to improve upon both the speed and reproducibility of chromatography, offering good resolution and the ability to predictably scale up. Fast flow resins also have extremely good flow properties and superior loading capacity, making it ideal for faster separation cycles, better purification, and lessened dilution.

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Applications of important lectins

Posted by The Protein Man on Jul 12, 2016 2:59:34 PM

“Lectin” word originated from latin word “legree” which means “to select”. Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins whose sugar specificities are unknown are called hemagglutinins. Lectins are present in every organisms and are involved in biological functions such as adhesive, defence against pathogens, immunomodulatory and regulatory.

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How to Determine the Chemical Solubility of a Product

Posted by The Protein Man on Jul 6, 2016 10:00:00 AM

It's often necessary to determine the solubility of a chemical before you can begin working with it. Chemicals, especially those used in labs, are often concentrated and distilled, in order to be easier to use, transport, and store. But these highly concentrated chemicals will have to be properly reconstituted if they are to be used reliably. The solubility of a chemical is its ability to dissolve when presented with a certain solvent. When it comes to chemicals, it's often important to know how soluble the product is and which solvents are most appropriate for its applications. 

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