The Protein Man's Blog | A Discussion of Protein Research

Cell disruption is a collection of techniques used for releasing biomolecules of interest from inside the cell.  This blog will discuss the various techniques which will be broken down into two categories, gentle and harsh cell disruption, there relative advantages and which tissue types work best with the particular technique.  

After running your gel and transferring your proteins to your membrane, there is one thing left for you to do to ensure the accurate analysis of your protein. You need to block all unoccupied sites on your membrane to prevent the non-specific binding of antibodies and other detection agents to your membrane during subsequent steps. If you take this step lightly, you'll risk compromising the reliability your results.

Sodium dodecyl sulfate or SDS, is an anionic surfactant (detergent) commonly used in research lab due to its multiple functions.  

Western blotting is an immunodetection technique used to first separate proteins by gel electrophoresis then to transfer those separated proteins from the gel matrix onto a secondary, more durable matrix, most commonly polyvinylidene difluoride (PVDF) or nitrocellulose.