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The Protein Man

The Protein Man

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Applications of important lectins

Posted by The Protein Man on Jul 12, 2016 2:59:34 PM

“Lectin” word originated from latin word “legree” which means “to select”. Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins whose sugar specificities are unknown are called hemagglutinins. Lectins are present in every organisms and are involved in biological functions such as adhesive, defence against pathogens, immunomodulatory and regulatory.

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How to Determine the Chemical Solubility of a Product

Posted by The Protein Man on Jul 6, 2016 10:00:00 AM

It's often necessary to determine the solubility of a chemical before you can begin working with it. Chemicals, especially those used in labs, are often concentrated and distilled, in order to be easier to use, transport, and store. But these highly concentrated chemicals will have to be properly reconstituted if they are to be used reliably. The solubility of a chemical is its ability to dissolve when presented with a certain solvent. When it comes to chemicals, it's often important to know how soluble the product is and which solvents are most appropriate for its applications. 

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ELISA Substrates: A Selection Guide

Posted by The Protein Man on Jun 29, 2016 10:00:00 AM

There are a wide variety of ELISA substrates designed for different systems and detection methods. These ELISA substrates are designed to detect alkaline phosphatase (AP) and horseradish peroxidase (HRP). In addition to their sensitivity, other factors, such as cost and ease of use, may also factor in. Choosing the right ELISA substrates begins with determining the limits of detection required. From there, a general type of ELISA substrate can be selected and the specific substrate can be narrowed down. There are five major types of substrate available: PNPP, ABTS, OPD, TMB, and ONGP. Of these, TMB is one of the most versatile. 

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Common Subcellular Markers You Need to Know About

Posted by The Protein Man on Jun 22, 2016 10:00:00 AM

Cells are made up of recognizable structures that each serve their own purposes and have their own characteristics. Common subcellular markers are often used to distinguish cells from each other and to identify potential irregularities within the cellular structure. Though there are a multitude of subcellular markers available, a few of them are more common and more easily identified than others.

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