Carbohydrate affinity chromatography is a method of choice for purification of glycoproteins, lectins and other carbohydrate metabolite proteins. This affinity chromatography uses carbohydrate ligands such as carbohydrates, glycoproteins and carbohydrate matrices for purification. The matrix needs to be activated for covalent immobilization of the ligand. Various methods used for the coupling of ligands depending upon the activating reagents are as follows:
Topics: Protein Purification
Gel filtration is a method of separating molecules by size. Among similar techniques, gel filtration is known as being a very simple and gentle procedure. When it comes to desalting and buffer exchange, gel filtration is often the preferred method as it can achieve better separation without any complex processes. Gel filtration is commonly used for chromatography, with the only negative being that it is very difficult to get high resolution results.
For the purposes of chromatography, fast flow agarose resin provides a stable, easy, and effective medium. Comprised of cross-linked agarose beads, fast flow agarose resin is able to improve upon both the speed and reproducibility of chromatography, offering good resolution and the ability to predictably scale up. Fast flow resins also have extremely good flow properties and superior loading capacity, making it ideal for faster separation cycles, better purification, and lessened dilution.
“Lectin” word originated from latin word “legree” which means “to select”. Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins whose sugar specificities are unknown are called hemagglutinins. Lectins are present in every organisms and are involved in biological functions such as adhesive, defence against pathogens, immunomodulatory and regulatory.
