The Protein Man's Blog | A Discussion of Protein Research

The Protein Man

The Protein Man

Recent Posts

What is Drop Dialysis: An Overview

Posted by The Protein Man on Sep 12, 2016 10:00:00 AM

After the preparation of DNA solutions, some of the enzymes involved may be impacted by the residues of the chemicals that have been used. This may include excess salt, SDS, or other inhibitory substances. In order to properly utilize the results of the DNA preparation, it is sometimes required to perform a drop dialysis. Ideally, the drop dialysis will be able to "wash" the enzymes in question, removing the residue and providing a better and more accurate result.

Read More

How to Ensure Proper Protein Transfer to a Membrane

Posted by The Protein Man on Aug 23, 2016 10:00:00 AM

Poor protein transfer can lead to either a weak signal or a lack of signal being observed during the process of western blotting. Though poor protein transfer does occur fairly often, it's also commonly disregarded as a potential cause for a failure. Consequently, it's an issue that should be considered first when trying to determine the cause of a poor signal. 

Read More

Role of Lytic Enzymes in Protein and DNA Extraction

Posted by The Protein Man on Aug 16, 2016 10:00:00 AM

Lytic enzymes have a fairly prominent role in protein and DNA extraction. There are multiple enzymes that can be potentially used for lysis, depending on the applications and your lab's unique needs. Understanding lytic enzymes is essential to the process of successful protein extraction. Though physical lysis has been used in the past, it's no longer feasible -- and it's always important that lysis be completed with the proper lytic enzymes, to yield the most valuable results. 

Read More

How to protect proteins during protein extraction

Posted by The Protein Man on Aug 1, 2016 12:30:42 PM

Proteins are highly heterogeneous, complex bio macromolecules consisting of one or more long chains of amino acids. Proteins or peptides fold up to form secondary and tertiary structures, and associate with other protein subunits to form quaternary structures. Proteins are structurally and functionally different from each other and require distinct surrounding environment for their stability and activity. Proteins are susceptible to degradation, denaturation and precipitation when taken out of their native environment.

Read More

Topics: Protein Purification, Protein Extraction

Want more Protein Man blogs?

Purification_resins
Ellyn Daugherty's Biotechnology: Science for the New Millennium