The Protein Man's Blog | A Discussion of Protein Research

How to we immobilize our antibody over a column to extract the antibody's specific antigen from different samples?

Traditional Immunoprecipitation procedure can be divided into four key steps (Figure 1):

The answer is simple. It really doesn't matter as they are in fact the same.

Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed.  Its brand name is derived from Separation-Pharmacia-Agarose.

Question:

How to choose affinity tags for recombinant protein purification?