The Protein Man's Blog | A Discussion of Protein Research

Basically, protein tags are peptide sequences that are attached to proteins to facilitate easy detection and purification of expressed proteins. In addition, they can also be used to identify potential binding partners for your protein of interest.

How to we immobilize our antibody over a column to extract the antibody's specific antigen from different samples?

 

Traditional Immunoprecipitation procedure can be divided into four key steps (Figure 1):

The answer is simple. It really doesn't matter as they are in fact the same.

Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed.  Its brand name is derived from Separation-Pharmacia-Agarose.