The Protein Man's Blog | A Discussion of Protein Research

While antibiotics are designed to directly hinder bacterial translation, their efficacy is significantly reduced when pathogens develop drug resistance through a variety of mechanisms, which include ribosomal modifications, translation elongation factors, ribosomal protection proteins, and mistranslation.

Proteinase K is a serine protease, the presence of a catalytic triad characterizes serine proteases, the catalytic triad is a cluster of three amino acids that make the catalytic center and consists of serine, aspartic acid, and histidine amino acids, which can often vary but all of these enzymes have a nucleophile serine and the same catalytic mechanism. Proteinase K has a catalytic triad consisting of Ser 224, His 69, and Asp 39. The substrate recognition sites are made up of peptide chains, 99-104 and 132-136.