Proteinase K is a serine protease, the presence of a catalytic triad characterizes serine proteases, the catalytic triad is a cluster of three amino acids that make the catalytic center and consists of serine, aspartic acid, and histidine amino acids, which can often vary but all of these enzymes have a nucleophile serine and the same catalytic mechanism. Proteinase K has a catalytic triad consisting of Ser 224, His 69, and Asp 39. The substrate recognition sites are made up of peptide chains, 99-104 and 132-136.
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Topics: Protein Purification, Molecular Biology
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Topics: Molecular Biology, Bioassays
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Topics: Molecular Biology, Cytotoxicity Assays, Bioassays
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Topics: Molecular Biology, Protein Electrophoresis, Protein Estimation, Assay Development (ELISA), Protein Concentration, Bioassays