The Protein Man's Blog | A Discussion of Protein Research

The Protein Man

The Protein Man

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Detergents and Proteins, a Refresher

Posted by The Protein Man on Feb 10, 2021 3:00:00 PM

Detergents are amphipathic compounds with a nonpolar, hydrophobic tail and a polar, hydrophilic head group. Due to these structural features detergents tend to aggregate into structures called micelles at high enough concentration; arranging themselves with their hydrophobic tails pointed inwards and their hydrophilic heads pointed outwards. Detergents come in three types: ionic (cationic and anionic) and non-ionic. Non-ionic detergents aren’t generally used for gel electrophoresis due to their limited ability to break non-covalent interactions between protein residues and inability to impart a uniform charge onto the protein. Ionic detergents (typically anionic SDS) are used for gel electrophoresis as they are highly useful for protein solubilization, linearization and for establishing a uniform charge in preparation for gel electrophoresis.

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Topics: Protein Purification, Western Blotting, Protein Estimation, Detergents, Sample Clean Up, Protein Concentration, Protein Fractionation, Protein Labeling, Protein Extraction, Protein Detection

Post-translational Control of protein abundance in mammalian cells using a tunable destabilizing domain from E. coli

Posted by The Protein Man on Feb 1, 2021 4:15:00 PM

 

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Parkinson's and a potential cure

Posted by The Protein Man on Jan 26, 2021 1:00:00 PM

The Potential of Neuronal Transplantation in Treating Parkinson’s Disease

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Topics: Molecular Biology, Sample Clean Up, Protein Concentration, Bioassays

The Role of the Toll (like receptor)

Posted by The Protein Man on Jan 19, 2021 4:00:00 PM

 

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Topics: Protein Estimation

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