I am looking for a stain to use on a Tris-Tricine polyacrylamide gel that will stain a small peptide (~3kDa) that has no positively-charged amino acids. It's a very acidic peptide consisting only of neutral and negatively-charged amino acids. Coomassie does not work. Thank you.
Topics: Protein Electrophoresis
I am needing a product to concentrate 20mg of protein. My current volume is 15mL and I need to get to under 5mL volume. I have used spin columns in the past and my protein has gotten stuck on the cellulose membrane, so I am looking for alternatives.
Topics: Sample Clean Up, Protein Concentration
Topics: Detergents, Protein Extraction
Proteomics is the analysis of a species' protein complement that is expressed by its genome, but the sheer complexity of the proteome raises significant issues for a researcher. There are an estimated 20,000-25,000 human protein-coding genes that could code for >1,000,000 proteins, especially when protein modifications are considered. These include N- and C-truncations, alternative spliced forms and the large number of Co- and Post-translational modifications, including:
Topics: Protein Fractionation
