Protein quantitation or estimation assays are widely used for determining protein concentration and are considered to be one of the most widely used methods in life science research. Estimation of protein concentration is necessary in protein purification, electrophoresis, cell biology, molecular biology, and other research applications.
Topics: Protein Estimation
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While protein assays have a variety of uses in life science research, there is no single assay method that is suitable for all applications. Despite all the advancements in modern science, a protein assay method that is not affected by any non-protein components or by the differences in protein composition does not exist. For this reason, protein laboratories find it necessary to have more than one type of protein assay for research applications.
Topics: Protein Estimation
Basically, spectrophotometry is one of the most widely used analytical procedures in biochemistry. It is commonly used to estimate the level of an analyte in solution and is ideal for simple routine determination of small quantities of materials. This method is based on the two laws of light absorption by solutions, namely Lambert's Law and Beer's Law.
Topics: Protein Estimation
How to Determine Molecular Weight of Protein with SDS-Page?
