Life scientists know the frustration of running PCR (Polymerase Chain Reaction), checking the product on a gel or sequencing, and discovering that the amplified product contains unwanted DNA sequence. A single base error or insertion can alter a reading frame, disrupt a protein’s function, and compromise downstream analysis and application. These issues can originate during amplification if the choice of DNA polymerase fails to provide the accuracy the application requires. Selecting the right DNA polymerase enzyme is critical for achieving accurate, reproducible amplification, especially when the application demands high fidelity.
