The Protein Man's Blog | A Discussion of Protein Research

Best Blocking Buffer Selection for ELISA & Western Blot

Posted by The Protein Man on Jan 10, 2017 8:02:17 AM

Antibodies are used to detect specific proteins during research. There are a variety of tests including the Western blot, that produce a surface area that can contain other proteins and components that need to be blocked. Blocking buffers are used to bind the surface areas that may attach to reagents during the testing process, preventing the non-specific antibodies from interacting with the reagents and causing a poor reading or inaccurate results. Knowing which buffers to use, however, is critical for ensuring the accuracy of a test, as each option has its own advantages and disadvantages.

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Topics: Western Blotting, Assay Development (ELISA), Protein Detection

How can the Vmax of an enzyme reaction be calculated from the colorimetric SAM assay?

Posted by The Protein Man on Jan 29, 2014 1:56:00 PM

To calculate the Vmax and Km of the reaction you will need to run various concentrations of the methyltransferase to be tested and calculate the activity at each concentration.

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Topics: Apoptosis Assays, Assay Development (ELISA)

What Assay Development Accessories Should You Use For Protein Binding? (Part 3 f 3)

Posted by The Protein Man on Jan 7, 2014 5:00:00 AM

Question:

What assay development accessories should be used for protein estimation?  

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Topics: Assay Development (ELISA)

What Assay Development Accessories Should You Use For Protein Binding? (Part 2 f 3)

Posted by The Protein Man on Dec 31, 2013 5:00:00 AM

Question:

What assay development accessories should be used for protein binding?  

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Topics: Assay Development (ELISA)

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