The Protein Man's Blog | A Discussion of Protein Research

Culturing cells in the dishes has been the most critical and an important tool in the field of drug discovery and development for decades. Cell culture based assays allow a simple yet cost-effective technique bypassing tedious and tiresome animal-testing. This also provides a controlled situation, which can provide results based on the responses of chosen cells due to any stimuli or drug. For the past few decades, many of the methods were developed keeping the conventional two-dimensional monolayer culture in consideration where a single layer of cells is grown on a flat surface having a matrix on it. Nonetheless, 2D culture came as a boon for cell-based assays, but it did have its own share of limitations. The most important of all is the inadequate consideration of in-vivo environment where the cells are surrounded by the extracellular membrane in a 3-dimensional manner while 2D cultures do not consider cell-cell interaction in a 3D fashion providing an incoherent data.

Molecular biology based diagnostics are gaining wide importance for detecting diseases. Molecular diagnostic kits are simple and can either be used by a professional healthcare personnel or by the patient at home. Point of care diagnosis is gaining importance as it is easy to use in remote places by individuals with less or no experience without the need of sophisticated equipment. This method is based on techniques like PCR, in situ hybridization, ELISA, etc., that detect biomarkers specific for that particular disease. Lateral flow assay is one of the point of care techniques used to detect human, animal and plant diseases, food testing or soil testing. Usually liquid samples like blood, urine, serum, milk etc., are suitable for testing by this method but solid sample like tissues, soil, food etc., can also be tested by mixing the samples in suitable buffers.

In in-vivo systems, the cells have mechanical support in extracellular vicinity that not only provides overall stability but also facilitates cell-cell interaction, adhesion, cell proliferation, migration and differentiation. This mechanical support is a complex functional bioactive scaffold called the Extracellular matrix (ECM).

Loop mediated isothermal Amplification (LAMP) technique is one of the recent PCR techniques that is gaining importance for its application in molecular diagnostics. LAMP is a single tube PCR reaction carried out at constant temperature. LAMP is simple, affordable, cost effective, requires simple machinery and can be used in remote places by persons with little training.