The Protein Man's Blog | A Discussion of Protein Research

Seven Quick Solutions to Protein Electrophoresis Loading Problems

Posted by The Protein Man on Feb 24, 2012 11:44:00 AM

Question

How can I prevent my protein samples precipitating when loading on to a polyacrylamide gel (SDS-PAGE)?

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Topics: Protein Electrophoresis

Protein Gel Staining: Detecting Small Peptides

Posted by The Protein Man on Feb 15, 2012 2:27:00 PM

Question

I am looking for a stain to use on a Tris-Tricine polyacrylamide gel that will stain a small peptide (~3kDa) that has no positively-charged amino acids. It's a very acidic peptide consisting only of neutral and negatively-charged amino acids. Coomassie does not work. Thank you.

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Topics: Protein Electrophoresis

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